Re: [Histonet] Unregistered HT

Ha, surely you kid! I didn't go into science for the money, but only because I love it. Even though I get paid horribly (seriously, I qualify for Medicaid when the government includes my husband's income!), I love being in a lab. This is with a BS in Microbiology. I try not to think about the mone

Re: [Histonet] Help

FACEPALM.JPG this is not even worth an actual jpg. sigh. "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Wed, Sep 11, 2013 at 7:5

Re: [Histonet] Unregistered HT

Seriously, I can go home?! I'm going to tell my boss that Sarah Dysart said I could go home. When she asks who that is, I'll just look at her and repeat your name. Slowly. And walk away. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow

Fwd: [Histonet] Unregistered HT

How do you gain credits? Do you get them for attending seminars or do you have to take actual courses? Are these the CME's I always see noted for lectures? I never knew what those were for. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Gro

Re: [Histonet] Unregistered HT

Do you get paid more with that title? Just curious, I'm not sure what "laboratory professional" means, as I'm not in that kind of lab. Or is it a respect thing? Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they

Re: [Histonet] Unregistered HT

lle weaver [mailto:joellewea...@hotmail.com] > *Sent:* Tuesday, September 10, 2013 2:59 PM > *To:* Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu > *Subject:* RE: [Histonet] Unregistered HT > > ** ** > > Well I am mostly clinical...but I think

Re: [Histonet] Unregistered HT

Do employers consider lab techs to be proficient enough? I've been doing ISH and immuno for 13 years, but I'm not certified as I do research. Maybe there aren't a lot of lab techs out there? Just wondering since you might be missing someone awesome by hiring only certified people. "By bitching an

Re: [Histonet] Unsubscribe, Chapter 195

Excellent idea!! "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Sat, Sep 7, 2013 at 4:57 PM, Amos Brooks wrote: > Hi, > I

Re: [Histonet] centrifuge?

is strictly prohibited . > If you have received this communication in error, please notify us > immediately by e-mail, and delete the original message. > > On Thu, Aug 22, 2013 at 10:30 AM, Emily Sours wrote: > >> I don't know how many of you use ultracentrifuges, but when

Re: [Histonet] Uncertified Histotechs

I've noticed that a few jobs at UPMC for histotechs require experience, but not certification. You do have to be certified within a year of employment though. I'm thinking this is due to the lack of research jobs, so people like me (lab techs from research) are moving into hospital labs with enou

[Histonet] centrifuge?

I don't know how many of you use ultracentrifuges, but when you move them, do they need to be recalibrated? I can't find anything on it, but I don't want to break it because I moved it around too much. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror

Re: [Histonet] histology humor on youtube

Hey, I just found that today too! Are you a farker?? I almost sent it to the list, but then decided someone had already done so and I missed it. I heard that the university is upset because it's gone viral, something about inappropriate behavior in the lab. NO HAVING FUN, EVER!! :) Emily "By b

[Histonet] a stretch

Does anyone know Tony Green at Presby UPMC? I can't find his contact info and UPMC doesn't let you search employees. And if you do, I LOVE YOU AND I NEED A JOB. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could the

Re: [Histonet] RE: Plastic/Plexiglass Incubation chamber for IHC slides

We use bioassay dishes with 10mL pipets glued to them. The slides sit on two pipets and you can get two rows of slides in. Cutting the pipets' ends off is the hardest part. I take that back, finding a glue that will hold the pipets has been a problem lately, as rubber cement isn't made like they

Re: [Histonet] (no subject)

No. "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Tue, Jun 18, 2013 at 11:18 AM, Ruth Riisnaes wrote: > Please remove me from t

[Histonet] antibodies online

Hello Histonetters! What search engines do you use to find antibodies that are available commercially? I use biocompare, but I'm not sure if that's the best site. I would just google it, but that brings up wy too many protocols and papers (though the papers are sometimes a good source as well

Re: [Histonet] staining near pap pen only????

on Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent

[Histonet] staining near pap pen only????

Hello Histonetters! We have been doing triple staining on frozen tissue for a while. Just recently it started to stain only near the pap pen (the sections have two lines of pap pen, one where they begin and one where they end). This makes no sense to me, as the staining isn't only on one end (as

Re: [Histonet] PAP Pen With Aqueous Mounting Media

We just leave the pap pen on. Since it's not covering the tissue, it won't matter. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunt

Re: [Histonet] Talk to me about shelf life and outdates

We have stains from at least twenty years ago, judging by their labels--they have no date on them! I always wondered how long neutral red lasts, but apparently, it lasts a long, long time. We are not a clincial lab though, we do research. Emily "By bitching and bitching and bitching, they could e

[Histonet] Re: pH and temperature

Well, I found this on the site below: To make an accurate pH measurement you need to use temperature compensation. Exception is if you measure a pH of about 7. Which makes me feel better. Every other solution I pH is made at room temperature. I don't know why our para precipitates out--it might b

[Histonet] pH and temperature

Hello! Can someone explain why pH is temperature dependent? Really, I'm just worried about pHing 4% paraformaldehyde at about 50 degrees Celsius. If I wait until it cools down to room temp, the para usually precipitates--I assume because the pH is off. I know I could measure the pH at room temper

Re: [Histonet] Medical Antiques Estate Sale

Wow, that is AWESOME!!! Wish I were closer to TN to go!! Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Fri, Feb 15, 2013

Re: [Histonet] SOX10

We can't get sox10 to work on anything, let alone with a processor. We use chick tissue though. Emily On Sep 19, 2012 3:57 PM, "Michael Kent" wrote: > Hello, > > > > Has there been a successful response to the question on SOX10 on Ventana > platform? We have very faint labeling on first tries.

Re: [Histonet] Frozen Sections Falling Off Slides

Wow you can run frozen sections through those machines? That's awesome. I figured it wouldn't be gentle enough to process them. I'm a fan of Fisher Scientific superfrost plus slides. Fisher claims the slides are electrostatically charged to keep the sections from falling off. Whether that's a bu

OT: Re: [Histonet] Armadillos

I bet guy named Terry found a baby armadillo by the side of the road and nursed it. Now it sleeps under his bed. His name is Felix. Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh On Mon, J

[Histonet] Your Request to Histonet

s off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh On Sat, Jun 9, 2012 at 2:40 PM, wrote: > Unsubcribe Me Now...!! > > > ----- Forwarded Message - > From: Emily Sours > To: Li

[Histonet] the case of the disappearing dna

This isn't really histology, more molecular biology, but I'm not aware of any good molecular biology lists ^_^ I recently started making RNA probes again (after about a year of not doing so). When I linearize plasmid DNA (usually pBS, smaller than 4kb total) and run it on a gel, the band is really

Re: [Histonet] RE: Unsubscribe Me

That's it, I have had it with these Mister Falcon unsubscribes on this Mother Father list!! We need to start screening, using a quiz that involves three questions: 1. How do you unsubscribe from the Histonet list? 2. Describe in less than 100 words how one would stop receiving emails from the Hist

Re: [Histonet] RE: Unsubscribe Me

Don't kid! This is the digital age, how can anyone not know how to unsubscribe themselves from a mailing list?! And speaking of being behind the times, I just found out my university's copying center SCANS hard copies of documents to print them in larger quantities. Apparently, they don't have tho

Re: [Histonet] Fluorescence-filters

Can you do fluorescence with LED? (That may be a stupid question, as I always thought LED was just for brightfield). Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh ___

Re: [Histonet] Fluorescence-filters

Wow, that was detailed and interesting!! I didn't know microscopes could even use LED, does that require a different setup, or just a different bulb? Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty

OT: [Histonet] View Transit of Venus

Also, the ISS will be taking close-up pictures of Venus, the first time the pictures will be very detailed as the cupola wasn't on the ISS before. http://www.flickr.com/photos/nasa_jsc_photo/7164346718/in/set-72157629649730820/ Emily "You see a peanut, day's off to a good start; you witness some

Re: [Histonet] Fwd: Plants

A canary? Well, I know what you used that for...how did that work out for you? Actually, I know the answer because you're alive. EH&S should really look into this option, screw the badges... Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a

Re: [Histonet] Frozen brains on cryostat

If they haven't been fixed, good luck! I've never been able to section unfixed tissue. Then again, I work with embryonic tissue only. Have you tried sectioning at a smaller section size? Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a h

[Histonet] looking for "chuck" for old rmc cryostat

Hello Histonetters! I am looking for a "chuck" (the metal disc you glue the block to) for an old RMC cryostat. Unlike newer cryostats, the piece that locks into the specimen head is a screw, not a thick base. Any idea where to get this? I haven't fully searched the internet but any help is appre

Re: [Histonet] Lets talk forceps

Roboz makes great forceps and they aren't expensive. I think Storz may have bought them out. Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that’s beautiful. --Ron Swanson O

Re: [Histonet] Need the name of a piece of equipment

Hey, when you find out the name, send it to the histonet list. I'm curious as to what it's called. The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that’s beautiful. --Ron Swanson On

Re: [Histonet] An immunologist...

Should I not send stuff like this, then? It's safe for work. Not for puns. The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that’s beautiful.

Re: [Histonet] Mouse abs on mouse tissue method

This doesn't really help solve your problem, but I have a question. Why are you using two secondary antibodies? Why not just use the conjugated one? Emily (starting Friday off right by eating candy for breakfast. I love Fridays!!) The whole point of this country is if you want to eat garbage,

Re: [Histonet] RE: Fixation for breasts

That subject heading is NOT safe for work. Even if you are talking about science. Emily (what? I know you were thinking it!!) The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that’s beau

Re: [Histonet] questionaire about fixation

appearance goes from the exterior to the interior of the tissue and that > advance is seen as a line, hence "the front line" of the penetration. > René J. > > --- On *Sun, 3/18/12, Emily Sours * wrote: > > > From: Emily Sours > Subject: Re: [Histonet] questionaire ab

Re: [Histonet] questionaire about fixation

What the heck is a frontline? I tried to google it, but I got nothing useful. The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that’s beautiful. --Ron Swanson On Sun, Mar 18, 2012 at 2

Re: [Histonet] anti-GFP staining rabbit polyclonal A11122

retrieval will not hurt the GFP antigen in formalin fixed tissue. > > Paula K. Pierce, HTL(ASCP)HT > President > Excalibur Pathology, Inc. > 8901 S. Santa Fe, Suite G > Oklahoma City, OK 73139 > 405-759-3953 Lab > 405-759-7513 Fax > www.excaliburpathology.com > >

Re: [Histonet] anti-GFP staining rabbit polyclonal A11122

We have never been able to get this antibody working with paraffin embedding on mice tissue. It works fine with frozen sections however. We always figured it was the heating that destroyed the GFP antigen. Paula, what fixation protocol and dilution do you use? Emily The whole point of this c

Re: [Histonet] Re: Does xylene cause skin cancer?

Really, the more important question is, if the lab is unwilling to provide PPE in this case, where else are they lacking in looking after your safety? I've never heard of using xylene without gloves (nitrile or latex). The EH&S department here at Pitt (academic research) would explode with horror

Re: [Histonet] Re: Histonet Digest, Vol 99, Issue 22

I wish I worked for a union because techs are paid very little to do a lot of work (research in academics). We're stuck in a pay range as well, which depends on how much education you've had. If you knew how little I make for what my responsibilities, no one would be taking my job. I do it becau

Re: [Histonet] PA area folks--company to NOT use

Am I the only one offended by Mr. Swanson's quote being used in a forum > for medical professionals? Just curious. > > > Sent from my Windows Phone > From: Rene J Buesa > Sent: 2/4/2012 9:12 AM > To: histonet@lists.utsouthwestern.edu; Emily Sours > Subject: Re: [Histone

[Histonet] PA area folks--company to NOT use

writing down a certain price on a purchase order. Just a warning. He works in the Pittsburgh area. I wish there was a yelp for scientists where I could post this, because I know a lot of people use him. Emily Sours The whole point of this country is if you want to eat garbage, balloon up to 600

Re: [Histonet] reagents without expiration dates

I've always wanted to have a contest to see who had the oldest reagents. My lab once had something that was 20 years old. Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that’s be

Re: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested

For myself, I can say this: I can give you the best sections you've ever seen on my cryostat. But when I tried to use a cryostat that had disposable blades (in which you have to pick the section up from a completely different angle), I was terrible at first. I think you really need to get a techn

Fwd: [Histonet] oct to paraffin

I may have missed this but does one fix the embryo again in Carnoy's or whatever after it has been washed (assuming it was fixed in the first place) I would think the initial para fixation would be enough and (again after washing in aqueous solution) dehydrating it up to xylene/toluene/non-aqeous s

Re: [Histonet] Music in the Laboratory

I am lucky enough to work in a closed lab setting so we can play whatever, until the boss comes in (she can't concentrate with music on). If I couldn't listen to something while sectioning, I think I might die of boredom. But forcing other people to listen to your music is never good, even when y

Re: [Histonet] Respirators and Routine Histology

When I work with xylene, it's always in the fume hood while wearing gloves. The same goes for formaldehyde. I never use a face shield for anything, I just do it in the fume hood if I need to use caution with a certain chemical. Emily The whole point of this country is if you want to eat garbage

Re: [Histonet] 2% low melting agarose gel

How is low melting point different than DNA grade? I assume it's cheaper, anything else? I know I could google it, but it's more fun to ask you guys. Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are fre

Re: [Histonet] unsubscribe

As much as I love your endearment, we hardly know each other! Link to unsubcribe is at the bottom of histonet emails The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that’s beautiful. --R

Re: [Histonet] (no subject)

Because your boss said to leave it at 84 degrees? Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that’s beautiful. --Ron Swanson ___

Re: [Histonet] paraffin temperature

Our paraffin melts at 60C with pressure from a vacuum. I don't know if there's actually enough pressure to affect the melting point of the paraffin, but we always check the thermometer hanging inside the oven to make sure it stays at 60C. If you have a certain brand you're not sure of, I would che

Re: [Histonet] pouring of liquid nitrogen into a cryostat

I would suggest dry ice (on top of something plastic on top of the metal shelves), NOT liquid nitrogen. Dry ice is usually cold enough to bring our cryostat to -28 C when it won't cool down past -24 C because of frost on the compressor. I don't think it actually makes the cryostat colder than tha

Re: [Histonet] RE: Re:peggy wenk comments on HT/HTL practical

I knew I couldn't get a job in a lab without experience, so I offered myself to anyone, anyone at all who was willing to teach me for free. Luckily I got an awesome job for a semester (volunteering) but it taught me a lot. You have to go out there and find yourself experience, you can't wait for i

Re: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin

How do you become a certified HT and not have any lab experience?! That's crazy. Not that i know anything about being an HT, but I'm a lab tech and I can't imagine going into the job never having been in a lab at all. What exactly do they teach you?! Emily A great book should leave you with man

Re: [Histonet] Mice embryo cryocuts

You need to leave the embryos in sucrose waaay longer than 5 hours. I would leave them at least overnight, if not more than that. You can probably take them out of the blocks and resink them in sucrose, if you're desparate. But next time, always sink (ie wash in sucrose) for at least overnight.

Re: [Histonet] Jill's MSDS question: a tech's perspective

I would respond to this by saying, if I were working with something that dangerous, I would know ahead of time what to do with it. I'm not going to spend any amount of time looking up an MSDS if something is on me RIGHT NOW, digital or not. Which is why I think it makes sense to have digital copi

Re: [Histonet] MSDS binders

ctual chemical name for things like TESPA. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Thu, Aug 25, 2011 at 5:16 PM, Emily Sours wrote: > I would say if your computer system is down, t

Re: [Histonet] MSDS binders

I would say if your computer system is down, then your electricity is probably out so I would hightail it out of there. Or ask yourself, why am I working with hazardous chemicals in the dark? Our EHS allows digital MSDS as well, but they say you could always call them if necessary. So maybe that'

Re: [Histonet] cadaveric bone

Wow, you section elephant tusk? That is so cool. And also, let me know what the answer is to your question, it's an interesting one. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Fri, Aug 1

Re: [Histonet] Aqueous mounting media

PM, Emily Sours wrote: > We use Gel Mount > > > A great book should leave you with many experiences, and slightly > exhausted. You should live several lives while reading it. > -William Styron > > > > On Tue, Aug 16, 2011 at 2:48 PM, Kevin Bennett wrote: > >>

Re: [Histonet] Aqueous mounting media

We use Gel Mount A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Tue, Aug 16, 2011 at 2:48 PM, Kevin Bennett wrote: > All, > Can anyone recommend a quality aqueous mounting media? I was using Aqua

Re: [Histonet] RE: H&E on Frozen

I concur on this. If my boss will let me, I can send you our elaborate protocol for H&E staining, It involves many dishes and many washes. But your H&E will be perfect. I don't have the protocol with me right now,as I'm at home but tomorrow I will ask about sending you the protocol. I don't see

Re: [Histonet] picture of gross lab senior

gross lab senior = obese elderly technician? i don't think s/he would appreciate you installing a keyboard on said person. emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Tue, Aug 9, 2011 at

Re: [Histonet] And now, IHC playing cards!

Hello again! I went to their site and can't find anything...hopefully I didn't miss it!! Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Wed, Jul 13, 2011 at 3:18 PM, wrote: > > > Those of

Re: [Histonet] And now, IHC playing cards!

Do you have to buy anything to get them? Or do you just go to their website? A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Wed, Jul 13, 2011 at 3:18 PM, wrote: > > > Those of you that like freebi

Re: [Histonet] microtome knife holder

All of the Leica cryostats have knife holders, I would contact Leica to ask as their website is terrible. You could also just google it and I bet some other company sells them. The other one, I'm not sure of. We don't use disposable blades so we've never had a knife holder for that. Emily A grea

Re: [Histonet] Could you please recommend? Cryostats.

You can't get any better than a Leica 3050. That is top of the line to me. Unless there's some really expensive brand out there I'm not aware of. I love our 3050. I guess your problem is you need a UV lamp, and the 3050 doesn't have one. I would say stick with Leica, their cryostats are awesome

Re: [Histonet] Frozen tissue question

>From the research point of view, I've heard of people not fixing tissue before they section it. Since I work with embryonic tissue (which is mostly water!), we always fix our tissue. It definitely is better to not fix tissue when you want to stain with antibodies because anything you do to the sam

Re: [Histonet] Retirement

Retirement? I think by the time I get to that point, social security will have run out. Then again, technology will be so advanced, I can tell stories about the old days, where I logged on to the bbs by modem to post messages to my friends and typed in my own html coding. We didn't have google when

Re: [Histonet] Re: Anatomicalalal

I'm going to start using this pronunciation. It's so hardcore! A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Mon, Jun 13, 2011 at 4:54 PM, Robert Richmond wrote: > > > "Periodic acid" is pronounc

Re: [Histonet] Light + IF microscope

Nikon is expensive, but they are very friendly and will fix little things for you for free (not that they'll tell you that upfront). Our Nikon representative is always coming by if we have even the slightest problem and is willing to figure out weird problems that come up. Also, they always check

[Histonet] endogenous peroxidases

Hello I know I can block endogenous peroxidases in paraffin sections before antigen retrieval or after it, but which is better? Does it make a difference? Also, since our H2O2 is 30%, do I need to account for the water in it when I'm diluting it in 100% MeOH? (I was going to use a 0.3% H2O2 in MeO

Re: [Histonet] How to keep paraffin sections from sticking to blade/blade holder.

Open a beer to the science gods. It may also help to follow the other instructions sent to the histonet list. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Thu, Apr 21, 2011 at 3:07 PM, Clo

Re: [Histonet] Unsubscribe

Jupiter's thunder two people in a row? really?!!?!? LOOK AT THE BOTTOM OF THE EMAIL. YOU UNSUBSCRIBE ON THE SAME PAGE THAT YOU SUBSCRIBED ON. Both of you are officially banned from the internet. Forever. Don't use it again. And don't EVER join a mailing list. EVER. Emily, who would love t

Re: [Histonet] in-situ hybridization

Roche Diagnostics has great DIG labels, antibodies and color reaction chemicals. It is a pain to just start up, you need to buy a lot of stuff for it. Could you use someone else's set up and pay for part of the chemicals? Emily A great book should leave you with many experiences, and slightly e

Re: [Histonet] SOX10 Antibody

We have tried rabbit anti SOX10 from Millipore and Santa Cruz. Neither were specific to chick tissue, even though they were supposed to be due to sequence homology. There's my absolutely not helpful at all contribution towards your question. Emily A great book should leave you with many experien

Re: [Histonet] Leica Cryostat Service Recommendations

We have the same cryostat, three years old and this is the first time anything has broken on it. The total cost for everything will be about $600 which is wy cheaper than a $4000 service contract (for one year!). Also we use our cryostat almost every day, all day. If you're using it that litt

Re: [Histonet] science for kids

gt; Emily, > > NSH has a coloring book that explains histology. > > > Andi Grantham > > > > On Apr 5, 2011, at 7:14 AM, Emily Sours wrote: > > > I'm going to visit my nieces this summer and they want to know what I do > at > > work. Does anyon

[Histonet] science for kids

I'm going to visit my nieces this summer and they want to know what I do at work. Does anyone know of a good book or website that explains DNA and/or histology for 4 to 6 year olds? Just thought I'd throw it out there, since googling it would yield way more than I can deal with. Emily A great bo

Re: [Histonet] cryostat repair--thanks!

ead, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > > On 4/1/2011 12:09 PM, Emily Sours wrote: > >> Thanks to a

[Histonet] cryostat repair--thanks!

Thanks to all who answered my email, I ended up calling Tech One Biomedical and they came right out. I recommend them to anyone who needs histology equipment repaired!! Emily ps. this is not an april fool's joke. pps. really! It has become almost a cliche to remark that nobody boasts of ignoranc

[Histonet] another money saving question

Is there any way to extend the life of PAP pens? Add a little xylene to the tip or soak it in something? I feel like they run out so fast. Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and p

[Histonet] cryostat repair

Our cryostat (CM3050S) is making a terrible noise when the fan stops running, yet I don't want to call Leica (the manufacturer) because they charge more money than anyone could ever afford. How would I go about finding someone who could repair a cryostat in Pittsburgh? I don't think it's the refri

Re: [Histonet] please remove me from list. Thank you.

No. And you're not welcome. It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins On Tue, Mar 22, 2011 at 9:34 PM, wrote: > > > Please

Re: [Histonet] Telepathology

Okay, it's Friday so I have to write that my first thought was telepathology? Is that like telekinesis? Like processing slides and coverslipping with your mind? Would this extend to sectioning to? How about pouring your much needed cup of coffee, or even making the coffee? Emily It has become alm

Re: [Histonet] cdj

Now I'm curious!! Please let us know what this mysterious processing is!! Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins On

Re: [Histonet] Archives

Honestly, I would just use google and search with histonet and whatever your looking for. Much easier! It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics.

Re: [Histonet] in situ - thanks & 1 more query

The best place to look for antibodies from different companies is biocompare.com. They have a separate antibody search engine. That said, I'm sure Roche Diagnostics or Invitrogen has one. Roche is the original maker of the DIG system (at least, I think they are). Also, Jackson Immunoresearch ha

Re: [Histonet] Stupid, stupid static!!

You know, all of it sounds so easy...unless you're cryosectioning!! Does putting a humidifier outside help with static in a cryostat? Because the static is driving me nuts. Why aren't there teams of people working on this issue? It keeps coming up, yet there is no one good answer, Calling all eng

Re: [Histonet] Stupid, stupid static!!

Target! They have frogs too! I enjoy the elephant design the best. Well, next to Hello Kitty, but that's not for everyone. *http://tinyurl.com/2frkw3r* Emily Writer Richard Suflet recommended drinking large doses of strong vinegar with fleas to cure the illnesses that resulted from swallowing t

Re: [Histonet] Re: if you could pick your own floor surface...

shag carpeting. and it would extend to the walls, up to the ceiling. Writer Richard Suflet recommended drinking large doses of strong vinegar with fleas to cure the illnesses that resulted from swallowing the horse-leeches that were common in drinking water. -Every Home a Distillery, Sarah Meacha

[Histonet] OT? protein translation music

http://bit.ly/gk2iaa In the style of the newer science raps, here is a 70's version of protein translation. The explanation goes until 3:40, the music starts afterwards. Who knew protein translation was so groovy? I wish I had an mp3 of this!! Emily "It was more probable that Lord Aylesford was

Re: [Histonet] 10% Neutral Buffered Formalin - Methanol?

A related question: I was making Serra's fix (which has no water in it) and I tried to use buffered formalin. This turned the solution white (which happens when Serra's has water in it). Another brand of formalin (which did not say it was buffered) worked fine. What's in the buffering that has wa

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