[Histonet] Xylene substitute

Hello all, Looking for information on Xylene substitutes. I have never used it before and wondered: 1. Is it cost effective? 2. How do you dispose of it? 3. Do you use it just like Xylene - just as many times, or change more often? 4. Can you recycle it? Thanks in advance, -- *Ms. Gareth B. Davi

[Histonet] EPA

I have read CAP accredited labs need an EPA number. Are all labs getting one, or have one, and how difficult was it to get one? -- *Ms. Gareth B. Davis, BS, HT, QIHC (ASCP)cm* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.uts

[Histonet] Tech component and professional component

Are there any labs that do the tech component only and have a different lab read the slides? We would like to discuss with rural labs, who are also CAP, how you handle the process. Please send contact info or call me at 928-248-5259. We spoke with CAP, but would like to see others do it. Thanks.

[Histonet] TC/PC component lab

Are there any labs out there that just do the technical component, just making the slides and send those slides to a pathologist at another lab to be read? And what would be the concerns with this situation? Can a medical director also be the laboratory director for the lab doing the technical sid

[Histonet] Laboratory Equipment services and supplies

Has anyone used Laboratory Equipment Services and supplies, LLC for their preventative maintenance? Just wondering if they are good. They sent me a quote that was really good and much less than what I have been quoted. Any feed back would be appreciated. Gareth -- *Ms. Gareth B. Davis*, B.S., HT

Re: [Histonet] Bleach Regs

Hi Maryann, Nosy Med techs. I have bleach under my sink and never had a CAP inspector say anything about it being there. On Thu, Jan 31, 2019 at 8:31 AM Maryann Deathridge via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello Histonetters! > Has anyone heard of a regulation by CAP, C

[Histonet] Eosin on biopsy tissue

Thanks for all the responses, I will keep the eosin in alcohol for now. Don't worry, I will follow all CAP rules. Thanks again. -- *Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 ___ Histonet mailing lis

[Histonet] Opinion on dye on biopsies

So, I work in a small GI lab, and I put Eosin in my first formalin on my processor. My biopsies are very small and this helps, somewhat, to see the specimens for embedding and cutting. But, unfortunately, the esophagus tissues do not absorb the eosin much. Anyway, the hospital lab I work, part-t

[Histonet] Fwd: Specimen's from hospital to lab.

I sent this question out last week, someone answered and I guess I accidentally deleted the email. So, if anyone remembers answering my question about private labs doing hospital outpatient biopsies, please email me again. Thanks, Gareth Davis -- Forwarded message -- From: Gareth D

[Histonet] Specimen's from hospital to lab.

Are there any labs that are private, but receive specimens from a hospital? If so, what kind of protocol or policy do you give the hospital to follow in order for them to submit specimens to the lab? Thanks, -- *Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 9

[Histonet] Backup Power Supply

Hello All, ​We need back-up power supply via CAP regulations. Besides a generator or a dedicated supply through a hospital back-up generator, what are labs using. We are a small lab located in a clinic. There is no back-up power supply in the clinic. Wondered if there was anything out there tha

[Histonet] Proficiency Testing for small labs

I was wondering what other small CAP accredited GI labs did for proficiency testing. The lab I work in only does H&Es, and two IHC stains (H. pylori and CD8). that's it. All the PT programs with CAP are overkill for what little we do. In the past I have sent slides reflecting my work to an outs

[Histonet] Now I have ribbons

Thanks to everyone for their response to my problem with cutting last week. Things are much better, getting ribbons, now that I have increased my angle to 8. The humidity in Yuma is now actually above 10%, so I really think that helps too. I did try trimming the blocks to sharper corners, but th

Re: [Histonet] No Ribbon

Yes, I use ice for my blocks and get them as cold as possible. It is a high profile blade holder. The paraffin is good for embedding and processing. I was told to use a low angle for Statcut blades, but I will try it at 10. Thanks -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroente

[Histonet] No Ribbon

I feel like I should be able to figure this one out, but I have been having trouble with getting a ribbon for a while now. I have a Leica Microtome RM2125 and I use either a AccuEdge blade (I get one block to cut then it starts to compress) or a StatCut blade (sections do not stick together at all

Re: [Histonet] solvent recyclers

I have a CBG recycler and it has never given me any problems in the two years I have been here. The last two labs I worked in (in the past 15 years) both had CBG recyclers and loved them. Gareth Davis On Fri, Mar 24, 2017 at 11:00 AM, Bob Richmond via Histonet < histonet@lists.utsouthwestern.edu>

Re: [Histonet] : Recycled reagents in VIP processor

Thank you for your perspective. All input is helpful in our decision. Gareth On Wed, Mar 1, 2017 at 11:32 AM, White, Lisa M. via Histonet < histonet@lists.utsouthwestern.edu> wrote: > We have recycled here for over 14 years. The cost of the recycling unit > is not small. It will take a while t

Re: [Histonet] Recycled reagents in VIP processor

tem. E-mail transmission > >> cannot be guaranteed to be secure or error-free as information could > >> be intercepted, corrupted, lost, destroyed, arrive late or incomplete, > or contain viruses. > >> The sender therefore does not accept liability for any errors or >

[Histonet] Great input on recycled reagents

Thank you so much. I will start using them on the processor, but start slowly. Then I can monitor to make sure no problems occur. I really appreciate all the responses. Thanks, -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 ___

[Histonet] Recycled reagents in VIP processor

Hi, I was always told not to use recycled reagents, i.e. Alcohol and Xylene, in processors. I am using a VIP 300, refurbished, and I would rather not use recycled reagents in it. But, during the last CAP inspection they suggested I use the recycled to save money. And now my administration wants

[Histonet] IHC Medicare policy

Okay, I know that for Medicare cases we cannot order Special or IHC stains upfront. They can be ordered after the pathologist reads the slide. But, what are labs doing for other insurances? The lab I work in only does H. Pylori and CD 8 on GI cases. I understand cancer cases are different. I

[Histonet] correction on CAP policy

After researching and calling CAP I have realized it is not a CAP policy for pathologist to order IHC/SS only after viewing the H&E. But, it is an active LCD. I am new to regulations in labs, being my first time to run one. Pardon my mistake and thanks for the responses. -- Ms. Gareth B. Davis

[Histonet] pathologist adding IHC stains

How are labs indicating, on reports, that the pathologist has ordered IHC stains after reading the H&E slides. With the new CAP rules regarding ordering IHC stains, i.e. H. pylori and others, that are usually ordered initially. I basically would like an example of wording for the reports, when the

[Histonet] Processing time for small biopsies

Hi, Recently I saw a post from someone looking for a protocol for processing small biopsies. I looked on the histonet search, but found that to be too time consuming to locate the post. Anyway, I hoping to get some opinions on good process times for small biopsies. I recently changed my paraffin

[Histonet] H.pylori question

Okay, so I know I have heard rumors about the H.pylori testing being affected by Medicare. We currently do the H. pylori IHC stain on all gastric biopsies. Has anyone heard that Medicare will change, and we will no longer be able to do it on all cases? The last lab I worked in, in Tennessee, sto

[Histonet] Low volume IHC instrument

What IHC platform would you choose if you only stained for CD8 and H. pylori? Ventana, Leica Bond, or Biocare? Or is there another I am missing? Familiar with Dako, and don't think it would suit our lab. Thanks again. -- Ms. Gareth B. Davis, HT, QIHC (ASCPcm) Yuma Gastroenterology Yuma, AZ 8536

[Histonet] Ventana Ultra vs. Leica Bond Max

I was interested in anyone's opinion on the new Ventana Benchmark Ultra and Leica's Bond Max. I currently use the Ventana Benchmark XT. Ventana wants us to upgrade to the Ultra. Because our contract will be up in a year, my manager and lab owners want me to check out other options. Hoping to ge

[Histonet] Transport training for formalin fixed specimens

Okay, is there a training course for just transportion formalin fixed specimens. CAP told me last year that anyone picking up specimens for us has to go through a training course. The inspector seemed to be more concerned with the employees knowledge of formalin and possible spills. So, does anyo

Re: [Histonet] Ventana/Roche H. pylori

Thank you to all who have been responding. Very helpful information. I have been seeing this problem for almost a year. We have contacted YRMC and know their option. Unfortunately, we are under contract. But, the previous lab I worked in had the same issue, but with DAKO. But, the debris showed

[Histonet] Ventana/Roche H. pylori antibody

Hi, I have read on the Histonet, in the past, that other labs are having trouble with the H.pylori antibody from Ventana/Roche. I get "debris" that looks like the H. pylori bug, except it appears to "float" above the section and not in the normal areas that H. pylori stains. Is anyone else still

[Histonet] Medical waste in Arizona

Does anyone know if Arizona considers the cassette lids, bio bags and kim wipes, used on water baths, as medical waste. The recent post about being dinged for not putting those in medical waste, was the first I heard of anything like that. But was hoping that was just California. -- Gareth B.