We are looking to purchase two new microtomes and were wondering on thoughts
and recommendations from the experience of the people that actually use them.
Pros and cons would be extremely valuable on ergonomics, reliability and
overall performance.
Sheila Herrington, RT
Technical Lead,
We found that if using the stronger proteases/enzymes for retrievals, there
tended to be more mast cell staining seen.
Sheila Herrington
Technical Lead - Histopathology and Immunohistochemistry
Kelowna General Hospital
2268 Pandosy Street, Kelowna, B.C. V1Y 1T2
250-862-4300 ext 7587 or 7510
We also have recently started to see this artifact more than ever before, and
nothing in our process has changed. We have tried everything to correct to no
avail. Wonder if it is possible to be a change in some type of supply, either
xylene or coverslipping film. Something has changed but am
I also have been having low temp errors. I use Benchmark Ultras and they are
not a batch stainer, so pads are heated independently. I noticed the staining
on the particular pad with the error was not quite to the usual standard so I
have stopped using that pad. Have contacted Ventana for a
I have been experiencing the same random over staining issues with WT1 as well.
I use the Benchmark Ultras with Ultraview. It started around the same time as
a new lot number changed a few months back. I have tried everything, but being
so random it is hard to find a solution.
Sheila
Not if it is just from processing. Eosin from processing will not affect any
IHC or Special stains. If it was HE stained you just need to take back through
Xylene, alcohol to water. The alcohol will remove some of the eosin, and the
IHC process will not be affected by the stains. It will
Are you using mold release? Sometimes that can cause what you are describing.
Sheila Herrington, RT (CSMLS)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bonnie
Gambichler
Sent: Tuesday, January 29,
