and a Modified
Goldner's to obtain all my bone related information. With that said, I have
also never used this stain on paraffin sections and would be interested in the
results if you try it.
Jack Ratliff
Date: Wed, 18 Feb 2009 07:29:39 -0500
From: sbr...@vetpathservicesinc.com
To: histonet
What is the tissue? What are your endpoints?
Date: Wed, 18 Feb 2009 16:45:46 -0500
From: sbr...@vetpathservicesinc.com
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sanderson's Rapid Bone stain
Hello, I posted earlier regarding the Sanderson's Rapid Bone stain. I forgot
working with paraffin.
Jack Ratliff
On Jan 30, 2009, at 8:47 AM, Markus F. Meyenhofer
mi...@superlink.net wrote:
Hello all.
Does Safranin O staining work on plastic sections (bone, decalcified)?
Any help would be appreciated.
Regards,
Markus
me know if you need anymore help!
Jack Ratliff
From: perro...@wanadoo.fr To: histonet@lists.utsouthwestern.edu Date: Mon,
12 Jan 2009 19:02:05 +0100 Subject: [Histonet] Undecalcified bone in MMA and
antigen retrieval (Neil Hand?) Hi all, I'm encountering problems while
trying to do
clarification.
Jack Ratliff
Date: Fri, 5 Dec 2008 15:14:07 -0500 From: [EMAIL PROTECTED] To:
histonet@lists.utsouthwestern.edu Subject: [Histonet] Polymerizing BIG
methyl methacrylate blocks Does anyone here have experience with large
PMMA blocks, about 150 to 200 ml volume each
Jim,
Is it possible for you to provide more information as to the type of specimen
you are working with and how thick you are cutting the sections?
Jack Date: Mon, 1 Dec 2008 16:26:31 -0600 From: [EMAIL PROTECTED] To:
histonet@lists.utsouthwestern.edu Subject: [Histonet] GMA embedded
Pamela,
I get my boxes (wooden - holds 100 slides) from Brain Research Labs and they
cost around $33.00 per box. The website is http://www.brainresearchlab.com/
Jack
Date: Tue, 2 Dec 2008 21:40:41 + From: [EMAIL PROTECTED] To:
histonet@lists.utsouthwestern.edu Subject: [Histonet]
Jim,
Here you go:
MMA Thin (5 microns) Sections
1) Deplastify with three changes of Xylenes @ 60C for 30-60 minutes (depending
on size of section). You can also use three changes of acetone @ RT for 15-30
minutes.
2) Hydrate tissue from ethanol to DI water (100% EtOH for 5 min, 95% EtOH
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