If anyone is still doing Influenza A IHC testing, could you share your
vendor source and catalog number with me? I'm looking to find a better
source than what I'm using right now. I don't do it very often (since we
have a PCR test) but once in awhile it's requested, and my old antibody
just
Hi Sandra,
Being a mouse monoclonal, it may not cross-react with canines/felines; the
epitope may be specific to human follicular cells only.
The polyclonal Thyroglobulin from Dako/Agilent works fine on all the
mammals I've tried it on so far (and a frog as well).
Regards,
Jan
Jan Shivers
Hi Lisa,
I just worked up an antibody from abcam; cat. # ab85554
Rabbit polyclonal
1:1200 (0.6ug/ml) -1:1600 (0.4 ug/ml)
Heat retrieval in pH 6.0 solution
45 min primary Ab, 45 min secondary Ab (polymer system), 15 min chromogen
(AEC+)
Regards,
Jan
Jan Shivers
Senior Scientist
IHC/Histology
Hi Sandy,
We cut CD31, CD45, CD45RA, CD117, CD204, and MUM1 controls fresh (same
morning as staining; case material as well).
We've never had any problems with Synaptophysin; it's always worked very
well on unstained controls that are up to a year old (stored in the fridge).
Best regards,
Jan
Hello,
It depends upon which 'red' chromogen you are using. There are several
with different chemical compositions, so you will need to look at the
Safety Data Sheets which pertain to the chemical you are using.
In my lab we use 3-amino-9-ethylcarbazole (AEC) which is considered
hazardous. It
Has anyone ever performed IHC on frozen sections of guinea pig tissue? I
am experiencing an enormous amount of bubbling when doing the peroxidase
blocking step, even though I'm only using a 0.3% concentration of H2O2.
And when I say 'enormous', I mean it's like continuous champagne bubbles
rising
Hi Sandy,
I get mine from CosmoBio, catalog # KAL-KT022. Needs heat retrieval and I
go 1 hr incubation in primary Ab at room temp.
Good luck,
Jan
Jan Shivers
Senior Scientist
IHC/Histology Section Manager
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333
Are there any veterinary labs that do Bordetella IHC and can accept tissue
from outside of the US? Asking for a colleague.
Thanks in advance.
Jan Shivers
Senior Scientist
IHC/Histology Section Manager
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333
Hello Paula,
This isn't an answer to your question, because I do use the Millipore
antibody (MAB805) and it works quite well on a Dako platform or done
manually. It requires enzyme digestion antigen retrieval. I use it at a
1:400 dilution (45 min) with a polymer detection system (45 min).
I'm
Does anyone perform EMA/MUC1 IHC on canine tissue? If so, what company is
your vendor? You can reply to me in a private message. Thanks in advance.
--
Jan Shivers
Senior Scientist
IHC/Histology Section Head
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
10 matches
Mail list logo