[Histonet] Type II-A Alpha amylase derived from Bacillis species

2010-10-13 Thread JanKeeping
I was wondering if anyone is using this enzyme. If so, where do you buy it? Is it extremely expensive? Could you share your procedure for preparation? Janet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Alpha amylase

2010-10-13 Thread JanKeeping
Sorry, I was referring to glycogen digestion and PAS staining. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] PAS staining

2010-10-06 Thread JanKeeping
Although I have allowed Schiff's and Periodic acid to room temperature, temperature may indeed be a factor. The ventilation system within this lab is extremely effficient and takes in a great deal of outside air. I am in Newfoundland, and lower outside temperatures are more common than we

Re: RE: [Histonet] PAS staining

2010-10-06 Thread JanKeeping
The interesting thing about the test for Schiff reagent is that both texts (Freida Carson's and Sheehan Hrapchak) both specify 37-40% formaldehyde. Hence my confusion re; the color development On Oct 6, 2010 3:24pm, gayle callis gayle.cal...@bresnan.net wrote: I was taught to avoid aqueous

Re: RE: [Histonet] PAS staining

2010-10-06 Thread JanKeeping
The interesting thing about the test for Schiff reagent is that both texts (Freida Carson's and Sheehan Hrapchak) both specify 37-40% formaldehyde. Hence my confusion re; the color development On Oct 6, 2010 3:24pm, gayle callis gayle.cal...@bresnan.net wrote: I was taught to avoid aqueous