[Histonet] cryosectioning hydrogel

I am having a difficult time sectioning hydrogel @ 10u on the cryostat. Does anyone have a helpful suggestion? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] automated H+E and cover slipper

Hi All, I will be trying out new H+E autostainers and cover slippers soon. Does anyone have a favorite, and why? Thanks for your responses. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/hi

[Histonet] JB fixative

Hi All, I use a Zinc fixative called JB fixitive for tissues that I want to stain for CD antigens(see attachment). Does anyone know if this fixative kills all microbes and prions? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists

[Histonet] viral inactivation with JB fixative

Histonetters, Does anyone know if Zinc Fixative(J B Fixative) inactivates viruses and prions? I need a reference and haven't found one in numerous literature searches. Our lab is concerned that there may still be active virus in zinc fixed tissue since it doesn't contain formalin. Thanks,

[Histonet] prion contaminated tissue processing

Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet