Re: [Histonet] Primary works with ABC but not IF

2008-12-10 Thread John Steel
Hi All, I do agree with titration - try this first.  You may consider pre-blocking before use of the primary antibody.  In my experience, tryamide amplification is expensive, and may not be necessary - put this on the back burner.  Not sure on your specific applications, but am always

[Histonet] Microwave Processors

2008-12-01 Thread John Steel
Hi All, I am very curious about microwave processing technology, as I am told that total processing time is reduced to hours, and could use your candid opinions!  Does anyone have experience with this specific technology?  I understand that there are several units out there, but performance is

Re: [Histonet] RE: Dako and Leica immunostainers

2008-11-22 Thread John Steel
, reproducibility, and reliability is the hallmark of research and IHC. Best regards, Jerry From: Larry Woody [EMAIL PROTECTED] To: John Steel [EMAIL PROTECTED]; Amos Brooks [EMAIL PROTECTED]; [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu histonet