[Histonet] Iron stain on Bone Marrow crush specimens

Hello, Would anyone be willing to share their protocol for performing iron stains on bone marrow crush specimens? We are trying to convert from doing the stain manually to automated on a Ventana Special Stainer using the iron stain kit from Roche. We are using the following protocol: 1.

Re: [Histonet] Cutting benchmarks

There is an extensive article in Annals of Diagnostic Pathology by Rene Buesa which is a little on the old side (2010) but still good. You can find it at www.sciencedirect.com title is Staffing benchmarks for histology laboratories. Hope this helps. Carole L

[Histonet] H Stainer?

Hello All, Our lab is replacing our well-loved and used Sakura Prisma stainer, and I would appreciate input (good or bad) about H strainers. We are considering a new Prisma, a Dako CoverStainer, a Leica Spectra, or a Fisher Gemini. Thank you in advance! Carole L Johnson, HT(ASCP)cm,

[Histonet] Changing to reagent alcohol

We have been using ethanol in our lab and would like to change to using reagent alcohol for the obvious reasons of cost and regulatory headaches. I have been tasked with creating a validation/verification process. Does anyone have any suggestions? Or better yet, information proving that we

[Histonet] Opening in great veterinary diagnostic lab

We have a day shift opening in our lab. Beautiful lab, great equipment, awesome pathologists. If you are interested, please follow this link for more information. http://www.nmda.nmsu.edu/humanresources/ Carole L. Johnson, HT(ASCP)cm, QIHC New Mexico Department of Agriculture Veterinary

[Histonet] CWD testing

I would like to hear from people who are doing CWD testing on biopsies (tonsil or rectal). Are you placing a disclaimer on results, and if so do you include the number of follicles present in the specimen? You can answer privately at: cjohn...@nmda.nmsu.edu

[Histonet] Thank you!

Thank you to all who have responded with suggestions for Factor VIII, and happy Friday Eve! Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be

[Histonet] BioCare Mouse-on-Farma AP-Polymer?

Hello and Happy (almost) Friday, I'm behind the curve, I'll admit it! If you have had experience with BioCare's farma detection, , (or canine/feline), I would appreciate your input. I'm staining porcine brain using Refine Red (Leica) with good results but would like to decrease my background

[Histonet] Handline paraffin

embedding, specifically related to paraffin hazards? Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New

[Histonet] Vet lab accreditation

Hello and Happy Almost Friday, This question is for the veterinary labs: Who is your accrediting body? Is it AAVLD, NAHLN and/or are you CLI, CAP, AABB, JCI ISO 15189 or Joint Commission? I appreciate your input. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture

[Histonet] Charging for IHC and special stains - veterinary

in the pathology report. This is in accordance with audits from an external (certifying) agency. How are other vet labs complying with this? Any suggestions are greatly appreciated. Happy Friday, Carole Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic

[Histonet] Buying service contracts

Hello and Happy Friday everyone! So I am looking at purchase of a cassette and slide printer and would like to know what you think about the value in purchasing additional year service contracts? Thanks! Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary

[Histonet] Rabbit-to-Rabbit IHC

Has anyone had experience with the Rabbit-to-Rabbit Blocking Reagent from ScyTek? Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand

[Histonet] Disopsal of containers?

to for an answer. Thanks in advance. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad

[Histonet] Another Tol Blue question

. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most

[Histonet] BS in Histology

who pass the credentialing exams have demonstrated a body of knowledge that deserves to be recognized and respected. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which

[Histonet] Charge for unstained slides?

Good morning all, Would anyone mind sharing what their lab charges for generating unstained slides? I have a researcher who has requested 40-some, and I want to make sure I'm being reasonable but not cheap. Thanks in advance! Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department

[Histonet] Tissue Processor Validation Procedure

with my equipment records. I hope this helps. Best regards, Carole Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand

[Histonet] Formic acid disposal?

Hello everyone and Happy New Year, Can anyone who is using formic acid in their lab tell me how they handle/dispose of it after use? I realize there is some variability by region, but any help would be appreciated. Thanks in advance. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico

[Histonet] Control strength?

Hello all, How are you documenting trending signal strength of your controls for special stains and IHC? I was asked this question during an audit and would appreciate your help. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services

[Histonet] rabbit to rabbit IHC

Is anyone using the rabbit to rabbit blocking reagent from ScyTek? I am trying to run tularemia antiserum on rabbit tissue. Thanks in advance! Carole Johnson, HT(ASCP)cm Histopathology Section Supervisor New Mexico Department of Agriculture Veterinary Diagnostic Services Confidentiality

[Histonet] IHC for plague and tularemia

Hi everyone, I would love to hear from anyone who is running plague and/or tularemia IHC. If you have a protocol you are willing to share and/or vendor information it would be greatly appreciated. Thank you in advance! Carole Johnson, HT(ASCP)cm Histopathology Section Supervisor New Mexico

[Histonet] Biossusa antibodies?

Hello all, Has anyone had experience with antibodies from Biossusa? I was looking for antibodies and came across this company but I am not familiar with them. Any help is appreciated. Happy Monday, Carole Carole Johnson, HT(ASCP)cm Histopathology Section Supervisor New Mexico Department of

[Histonet] Amyloid Precursor Protein (APP) by IHC

Hello All, Does anyone out there in Histoland have experience with this antibody? I have been asked to look into running IHC on pig brain on the Bond Max platform. I am looking into the antibodies from LS Bio and Invitrogen/LifeSciences, and would appreciate any insight you could provide.