[Histonet] Cutting paraffin sections...on a cryostat?

2013-06-14 Thread Johnson, Kevin
Hi, all. A bit of an odd question: a colleague knows of someone wanting to cut paraffin sections who has a cryostat, but no microtome. Since a cryostat's basically a microtome in a freezer chamber, I thought that it may be awkward, but theoretically doable once it was brought to room temp and d

[Histonet] Could these samples have been saved?

2013-02-08 Thread Johnson, Kevin
Dear all, During an overnight tissue processing cycle, a malfunction occurred such that the sample basket was suspended in mid-air for several hours at probably the worst spot in which to do so---after the final absolute ethanol of the dehydration series. I continued the process manually in the

[Histonet] Plain Vanilla Autostainer?

2010-10-19 Thread Johnson, Kevin
I am a simple man in a simple lab with simple needs. I work in a multiple-lab research environment, typically providing paraffin/frozen sections + H&E to individuals who then perform their own manual IHC. A simple man, yes, but also a busy man, who needs to be freed from staining dishes in ord