RE: AW: [Histonet] overfixation with formalin C.Fox pdf article

Thanks Joelle. I took it from your block. Regards, José Luis Dr. José Luis Palazón Fernández Instituto de Investigaciones Científicas Universidad de Oriente Boca del Rio-Isla Margarita-Venezuela Direccion actual: Instituto de Ciencias Marinas de Andalucia-CSIC Campus universitario Rio San

[Histonet] softening agent for tissues embedded in paraffin blocks

Dear List-members I'm trying to cut some fish muscle samples (along with skin and some bone tissues) embedded in paraffin (fixed in Bouin's fixative and decalcified with EDTA). I find it very difficult to cut these blocks and I would like to know, based in your experience, what tissue

[Histonet] automatic processor solutions renewal

Hello Histo-fellows I would like to ask you how often you change the solutions on the tissue processor?. Do you change it after you have processed a certain amount of samples? or use other criteria to do decide when to do the changes? Many thanks in advance for sharing the tips. José Luis

[Histonet] EDTA

Dear List members Greetings I would like to ask you some questions about the use of EDTA as demineratizing agent. I have to do histology and histochemistry (including metallothioneins) in fish larvae preserved in Bouins solution. 1) First of all I would like to know what is the best formula

[Histonet] paper

Dear histo-fellows greetings I would like to know if any of you can please read a paper I wrote about immunohistochemistry of a fish islet. English is not my mother languaje so I would appreciate if any of you (whose mother languaje is english) can read it and make all comments you think