We buy Diff Quik II by the gallon directly from Dade. You don't need the kit. Judy Keller, HTL South County Hospital Wakefield, RI
________________________________ From: histonet-boun...@lists.utsouthwestern.edu on behalf of histonet-requ...@lists.utsouthwestern.edu Sent: Fri 6/19/2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 67, Issue 19 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Synaptophysin (Benny Babinsack) 2. biocare bcl 6 (Orr, Rebecca) 3. Cytokeratin 18 M30 apoptosis staining (Popp, Laurie A.) 4. RNA In Situ Hybridizaton (Bell, Pat) 5. FW: Cytokeratin 18 M30 apoptosis staining (Popp, Laurie A.) 6. Glypican-3 on BondMax (Steven Wilkes) 7. Glypican-3 on BondMax (Steven Wilkes) 8. Glypican-3 on BondMax (Steven Wilkes) 9. xylene and frozen sections (Lynn Dike) 10. Temp work (Loralei Dewe) 11. Diff Quik II (Jessica Piche) 12. reticulin stain (Derek Papalegis) 13. reticulin stain (Rene J Buesa) 14. RE: Diff Quik II (Jodie Robertson) 15. Re: anti Brdu (Reynolds,Donna M) 16. Ventana Reagent Cost?? (Tiana Fountain) 17. Good Used Equipment for Sale (Denise Van Eaton) 18. Mouse necropsy question (Thach, Dzung (NIH/NIAID) [E]) 19. Re: Ventana Reagent Cost?? (Rene J Buesa) 20. RE: Ventana Reagent Cost?? (Burton, Lynn) ---------------------------------------------------------------------- Message: 1 Date: Mon, 15 Jun 2009 11:54:04 -0400 From: Benny Babinsack <bbabins...@genesishcs.org> Subject: [Histonet] Synaptophysin To: histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> Message-ID: <of8f32beea.eb47166f-on852575d6.00575917-852575d6.00575...@genesishcs.org> Content-Type: text/plain; charset="ISO-8859-1" We use a IVD from Cellmarque and use pa ncreas for the control Benny Ba Lab Services Senior Tech,&n (740)454-5587 ***Confidentiality Notice*** The information contained in this e-mail message ([Histonet] Synaptophysin), including any attachments, are intended only for use of the individual or entity named above (histonet@lists.utsouthwestern.edu). This e-mail may contain information that is privileged, confidential and/or otherwise exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, any disclosure, dissemination, distribution, copying or other use of the communication or its substance is prohibited. If you have received this e-mail in error, please reply to this e-mail indicating you are not the intended recipient and immediately destroy all copies of this e-mail. Receipt by anyone other than the intended recipient is not a waiver of any privileged information. ------------------------------ Message: 2 Date: Tue, 16 Jun 2009 12:29:47 -0500 From: "Orr, Rebecca" <r...@northshore.org> Subject: [Histonet] biocare bcl 6 To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Cc: "'jpet...@bostwicklaboratories.com'" <jpet...@bostwicklaboratories.com> Message-ID: <b44ca3426a2c084f9ba90b93d528159be4e2c...@exch34.enhnet.org> Content-Type: text/plain; charset="us-ascii" Message: 3 Date: Mon, 15 Jun 2009 15:34:33 -0400 From: "Justin Peters" <jpet...@bostwicklaboratories.com> Subject: [Histonet] BCL-6 To: <histonet@lists.utsouthwestern.edu> Message-ID: <24d22de9e488aa43bf92a4389f2ddb1f05dfa...@mail1.bostwick.com> Content-Type: text/plain; charset="us-ascii" I am having trouble getting bcl-6 antibody to work (Biocare CM223). Does anyone have a good protocol for me to try? Hi Justin, I run BCl-6 from concentrate 1:50 with Biocare's RED diluent with a Decloaker using RED diluent. Most antibodies perform well with the "green" diluent; this particular antibody is best using the "RED". I also use Mach4 detection... if problems persist, contact me offline, I may be able to help with some other suggestions. Hope this helps. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 ********************* ------------------------------ Message: 3 Date: Tue, 16 Jun 2009 15:59:46 -0500 From: "Popp, Laurie A." <popp.lau...@mayo.edu> Subject: [Histonet] Cytokeratin 18 M30 apoptosis staining To: <histonet@lists.utsouthwestern.edu> Message-ID: <1488342adb74ec4e969c48bdcde0bd98fb7...@msgebe23.mfad.mfroot.org> Content-Type: text/plain; charset="US-ASCII" Has anyone worked with this particular antibody? Can you give me any advise as far as what worked? I have an investigator that wants to use this antibody in diseased liver tissue and nothing that I am trying is working. Thanks! Laurie Laurie Popp, BA HT ASCP cm TACMA Shared Services Mayo Clinic-Rochester, MN ------------------------------ Message: 4 Date: Tue, 16 Jun 2009 15:05:41 -0600 From: "Bell, Pat" <pat.b...@ucdenver.edu> Subject: [Histonet] RNA In Situ Hybridizaton To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <64db27005e2fd3439e88502d7a5c9121877cb00...@cortez.ucdenver.pvt> Content-Type: text/plain; charset="us-ascii" My supervisor wants me to learn In Situ Hybridization using RNA probes in formalin fixed, paraffin embedded tissues. I have a Dako Autostainer, but I know that some of the procedure would be manual. I would appreciate any protocols that would help me. Thank you for your help. Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. Aurora, Co. 80045 303-724-6077 pat.b...@ucdenver.edu<mailto:pat.b...@ucdenver.edu> ------------------------------ Message: 5 Date: Wed, 17 Jun 2009 12:08:08 -0500 From: "Popp, Laurie A." <popp.lau...@mayo.edu> Subject: [Histonet] FW: Cytokeratin 18 M30 apoptosis staining To: <histonet@lists.utsouthwestern.edu> Message-ID: <1488342adb74ec4e969c48bdcde0bd98fb7...@msgebe23.mfad.mfroot.org> Content-Type: text/plain; charset="US-ASCII" > Has anyone worked with this particular antibody? Can you give me any > advise as far as what worked? I have an investigator that wants to > use this antibody in diseased liver tissue and nothing that I am > trying is working. > > Thanks! > Laurie > > Laurie Popp, BA HT ASCP cm > TACMA Shared Services > Mayo Clinic-Rochester, MN > > ------------------------------ Message: 6 Date: Thu, 18 Jun 2009 15:38:22 -0400 From: Steven Wilkes <srwil...@gmail.com> Subject: [Histonet] Glypican-3 on BondMax To: histonet@lists.utsouthwestern.edu Message-ID: <8e5827cf0906181238x6ee4b038hc98ea547ee29...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters! Has anyone been using Glypican-3 on the Leica BondMax platform? If so, please msg me, as I have some questions. I am having a tough time finding the right conditions... dilution, pretreatment, incubation times etc.. thanks Steven srwil...@gmail.com ------------------------------ Message: 7 Date: Thu, 18 Jun 2009 15:42:12 -0400 From: Steven Wilkes <srwil...@gmail.com> Subject: [Histonet] Glypican-3 on BondMax To: histonet@lists.utsouthwestern.edu Message-ID: <8e5827cf0906181242nf88a092o4a3868a7b4ba3...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters! Has anyone been using Glypican-3 on the Leica BondMax platform? If so, please msg me, as I have some questions. I am having a tough time finding the right conditions... dilution, pretreatment, incubation times etc.. thanks Steven srwil...@gmail.com ------------------------------ Message: 8 Date: Thu, 18 Jun 2009 15:39:27 -0400 From: Steven Wilkes <srwil...@hotmail.com> Subject: [Histonet] Glypican-3 on BondMax To: histology jobs <histonet@lists.utsouthwestern.edu> Message-ID: <bay101-w5061a0061cca94443ab33ac1...@phx.gbl> Content-Type: text/plain; charset="Windows-1252" Hi Histonetters! Has anyone been using Glypican-3 on the Leica BondMax platform? If so, please msg me, as I have some questions. I am having a tough time finding the right conditions... dilution, pretreatment, incubation times etc.. thanks Steven srwil...@gmail.com _________________________________________________________________ Bing(tm) brings you maps, menus, and reviews organized in one place. Try it now. http://www.bing.com/search?q=restaurants&form=MLOGEN&publ=WLHMTAG&crea=TEXT_MLOGEN_Core_tagline_local_1x1 ------------------------------ Message: 9 Date: Thu, 18 Jun 2009 19:47:14 -0500 From: Lynn Dike <lynn...@hotmail.com> Subject: [Histonet] xylene and frozen sections To: <histonet@lists.utsouthwestern.edu> Message-ID: <blu129-w6d6ef256f9f660349b97abf...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Lynn _________________________________________________________________ Insert movie times and more without leaving Hotmail®. http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutorial_QuickAdd_062009 ------------------------------ Message: 10 Date: Thu, 18 Jun 2009 18:30:09 -0700 From: Loralei Dewe <lld...@gmail.com> Subject: [Histonet] Temp work To: histonet@lists.utsouthwestern.edu Message-ID: <7173d3c00906181830o27dfc5d3sbd8cc8d505006...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi everyone, I am looking for temp work. If you have availability on a travel basis let me know. I have 10+ years experience with histology and IHC in a research venue and am published! Cheers, Loralei ------------------------------ Message: 11 Date: Fri, 19 Jun 2009 05:51:58 -0700 (PDT) From: Jessica Piche <jessgro...@yahoo.com> Subject: [Histonet] Diff Quik II To: histonet <histonet@lists.utsouthwestern.edu> Message-ID: <150374.59014...@web82008.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Good Morning! I was wondering who out there is using the Diff Quik II stain for H. pylori? I am having a hard time finding where to buy it! Do you just buy the whole DQ kit or do you just buy Diff Quik II solution? Any suggestions would be great! Thanks! Happy Friday! Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital ------------------------------ Message: 12 Date: Fri, 19 Jun 2009 09:11:05 -0400 From: Derek Papalegis <derek.papale...@tufts.edu> Subject: [Histonet] reticulin stain To: histonet@lists.utsouthwestern.edu Message-ID: <4a3b8e69.3090...@tufts.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I am having some difficulty getting the proper results from my reticulin stain. I have been following and tweaking the procedure outlined in Carson's book without any success. Could someone please forward me their procedure that has all the kinks worked out? Thanks, Derek -- Derek Papalegis HT (ASCP) Senior Histology Technologist Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 ------------------------------ Message: 13 Date: Fri, 19 Jun 2009 06:26:34 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: [Histonet] reticulin stain To: histonet@lists.utsouthwestern.edu, Derek Papalegis <derek.papale...@tufts.edu> Message-ID: <431812.3131...@web65714.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Derek: I am sending it to you under separate cover. René J. --- On Fri, 6/19/09, Derek Papalegis <derek.papale...@tufts.edu> wrote: From: Derek Papalegis <derek.papale...@tufts.edu> Subject: [Histonet] reticulin stain To: histonet@lists.utsouthwestern.edu Date: Friday, June 19, 2009, 9:11 AM I am having some difficulty getting the proper results from my reticulin stain. I have been following and tweaking the procedure outlined in Carson's book without any success. Could someone please forward me their procedure that has all the kinks worked out? Thanks, Derek -- Derek Papalegis HT (ASCP) Senior Histology Technologist Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Fri, 19 Jun 2009 06:49:52 -0700 From: "Jodie Robertson" <jrobert...@pathologysciences.com> Subject: RE: [Histonet] Diff Quik II To: "Jessica Piche" <jessgro...@yahoo.com>, "histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <518cd6920aa7154193cbe5977cd880733a8...@psmgsrv2.psmg.local> Content-Type: text/plain; charset="iso-8859-1" I purchase Diff Quik Solution II in a 2.5L bottle from Cardinal Health Catalog # B4132-12A. It's not something that they have in stock and it usually ships from Dade Behring Inc. which is who makes it. It usually takes me a couple of weeks to get it, but I have no problem receiving it. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Friday, June 19, 2009 5:52 AM To: histonet Subject: [Histonet] Diff Quik II Good Morning! I was wondering who out there is using the Diff Quik II stain for H. pylori? I am having a hard time finding where to buy it! Do you just buy the whole DQ kit or do you just buy Diff Quik II solution? Any suggestions would be great! Thanks! Happy Friday! Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Fri, 19 Jun 2009 09:28:09 -0500 From: "Reynolds,Donna M" <dreyn...@mdanderson.org> Subject: [Histonet] Re: anti Brdu To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <785bbf0c5f49ce41ba74460a43a08f021449221...@dcpwvmbxc0vs3.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" We have used mouse anti-Brdu from Becton Dickinson for years. It is still available from BD BioSciences Cat. 347580. I know it is made in mouse but we used an IgG1 secondary and always had good results. There may be some cytoplasmic label, probably background, but you are interested in the nuclear staining and it is very distinct. We have used it on both frozen and paraffin processed tissues. Donna Reynolds, HT (ASCP) Dept. Cancer Biology U.T. M.D. Anderson Cancer Center, Houston Tx Message: 3 Date: Wed, 17 Jun 2009 14:26:09 -0400 From: "Madary, Joseph" <mada...@medimmune.com> Subject: [Histonet] source for rat anti mouse brdu? To: <histonet@lists.utsouthwestern.edu> Message-ID: <e5b1c3d1305efd4c9ca7f63094135f5e0226a...@md1ev002.medimmune.com> Content-Type: text/plain; charset="us-ascii" I have not done a brdu on mouse in five years. Used Harlan but they are not selling that anymore. I used a procedure using rat anti mouse brdu primary, rabbiot anti rat mouse adsorbed secondary, then ABC/DAB. Anyone have any idea on a good source? I used biocompare and found stuff but would like to hear comments. Also anyone use conjugated brdu? Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) ***************** ------------------------------ Message: 16 Date: Fri, 19 Jun 2009 10:42:09 -0500 From: "Tiana Fountain" <tfount...@exchange.hsc.mb.ca> Subject: [Histonet] Ventana Reagent Cost?? To: <histonet@lists.utsouthwestern.edu> Message-ID: <bf2f7b1bbd30c5478ef41cf80164d99c7a7...@hscxmsmx0010.ad.wrha.mb.ca> Content-Type: text/plain; charset="us-ascii" Hello everyone, I am looking for a cost of some of the Ventana reagents can anyone help? Specifically: LCS: CC1: CC2: EZ Prep: Reaction Buffer: I am not currently a customer so I didn't want to phone Ventana directly if I don't have to. Thanks Tiana -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. ------------------------------ Message: 17 Date: Fri, 19 Jun 2009 10:52:15 -0500 From: Denise Van Eaton <ds...@hotmail.com> Subject: [Histonet] Good Used Equipment for Sale To: histonetmailinglist <histonet@lists.utsouthwestern.edu> Message-ID: <snt105-w31d98abb65544f42a33748ae...@phx.gbl> Content-Type: text/plain; charset="Windows-1252" Good Morning HistoNetters- Good Used equipment for sale! We have a Leica TP1050 bench model that is currently being used on a daily basis. The 300 cassette capacity retort, will process with or without heat, with or without pressure or vacuum. It has the reagent management system to assist with "rotation" of reagents as well as the xylene extraction cycle for the paraffin. We purchased it "used" about 2 years ago and it has worked very well for us. We just need the space more than the additional processor. We also have a 4+ year old Dako Autostainer. Each run will hold 44 slides. It is an open system, and so will allow you to use whatever reagents you choose. This unit has worked flawlessly for us, but we are discontinuing our on-site IHC program. Both of these units have been well maintained, are sold "as is" and will need to be picked up by the purchaser. We have no shipping crates and/or the muscles to pack up either machine. We are located just east of the Kansas City, Missouri area in Blue Springs. Anyone interested, please contact Denise Van Eaton @ dvanea...@littonlab.com _________________________________________________________________ Hotmail® has ever-growing storage! Don't worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009 ------------------------------ Message: 18 Date: Fri, 19 Jun 2009 11:56:27 -0400 From: "Thach, Dzung (NIH/NIAID) [E]" <thac...@niaid.nih.gov> Subject: [Histonet] Mouse necropsy question To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <c6612d6b.d07%thac...@niaid.nih.gov> Content-Type: text/plain; charset="iso-8859-1" Hi Everyone!! I am collecting spleen, brain, and spinal cord from virus infected mouse. I will perfuse mouse with PBS. Do you think I need to use a new pair of scissors and forceps for each tissue in each mouse and between each mouse? Thanks, Dz ------------------------------ Message: 19 Date: Fri, 19 Jun 2009 08:57:20 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Ventana Reagent Cost?? To: histonet@lists.utsouthwestern.edu, Tiana Fountain <tfount...@exchange.hsc.mb.ca> Message-ID: <737537.89352...@web65709.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 `If you don't use their auto stainer probably you will not be able to use their antibodies that, as far as I know, are "optimized" to be used in their instrument in what they call "liquid cover slips" for room and high temperature. René J. --- On Fri, 6/19/09, Tiana Fountain <tfount...@exchange.hsc.mb.ca> wrote: From: Tiana Fountain <tfount...@exchange.hsc.mb.ca> Subject: [Histonet] Ventana Reagent Cost?? To: histonet@lists.utsouthwestern.edu Date: Friday, June 19, 2009, 11:42 AM Hello everyone, I am looking for a cost of some of the Ventana reagents can anyone help? Specifically: LCS: CC1: CC2: EZ Prep: Reaction Buffer: I am not currently a customer so I didn't want to phone Ventana directly if I don't have to. Thanks Tiana -----Inline Attachment Follows----- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destiné à la personne ou aux personnes à qui il est adressé. Il peut contenir des informations privilégiées ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autorisée est strictement défendue. Si vous n'êtes pas le destinataire de ce message, veuillez en informer l'expéditeur immédiatement et lui remettre l'original. -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Fri, 19 Jun 2009 11:46:37 -0500 From: "Burton, Lynn" <lynn.bur...@illinois.gov> Subject: RE: [Histonet] Ventana Reagent Cost?? To: "Tiana Fountain" <tfount...@exchange.hsc.mb.ca>, <histonet@lists.utsouthwestern.edu> Message-ID: <b34ed7468185b44ab6cecf566b1960d001d87...@il084ex110.illinois.gov> Content-Type: text/plain; charset="iso-8859-1" The prices I have are on the list you sent. I couldn't find the CC2 price quickly. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-boun...@lists.utsouthwestern.edu on behalf of Tiana Fountain Sent: Fri 6/19/2009 10:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Reagent Cost?? Hello everyone, I am looking for a cost of some of the Ventana reagents can anyone help? Specifically: LCS:$98.40 CC1:$565.80 CC2: EZ Prep:$37.80 Reaction Buffer:$43.80 I am not currently a customer so I didn't want to phone Ventana directly if I don't have to. Thanks Tiana ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 67, Issue 19 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet