[Histonet] Qiagen Qproteome FFPE Tissue Kit

2017-10-17 Thread Kalleberg, Kristopher via Histonet
Hello, Has anyone tried the Qproteome FFPE Tissue Kit kit from Qiagen: Qproteome FFPE Tissue Kit For isolation of full-length proteins from formalin-fixed tissues * Standardized, quantitative extraction from FFPE tissue * Full-length proteins * Low sample requirements The

[Histonet] COL3A1

2017-03-22 Thread Kalleberg, Kristopher via Histonet
Hello, All, Curious if anyone knows of a great COL3A1 (Collagen III) antibody that works well in IHC for paraffin sections for human skin. I have tested a few and the ones that work/stain seem to stain most of the dermis of the human skin and I am concerned that they are staining for other

[Histonet] keratohyalin granules

2016-02-17 Thread Kalleberg, Kristopher via Histonet
Hello All, Does anyone have experience staining the keratohyalin granules in skin with different types of hematoxylins? I am currently looking at keratohyalin granules and staining with Harris hematoxylin and there seems to be little staining and not as prominent as I would have expected.