Hi Hazel
We use INI-1, clone 25/BAF47, BD Transduction 612110. Working conc. is 2.5 mg
Ig/l (1:100), HIER at high pH with a somewhat strange buffer (800 mM urea, 100
mM Tris, pH 9.5), but you may try EDTA-based HIER solutions, pH 8.8-9.5.
Incubation at RT, 60 min. Visualization with
We have ours from Thermo Scientific, CatNo MS-1081, same clone (4A4), works
very well (dilution 1:400 in our hands).
Andi Kappeler
Institute of Pathology, University of Bern, Switzerland
Von: histonet-boun...@lists.utsouthwestern.edu
