[Histonet] Help: IHC service

HELP: Hi, Is there anybody can provide me the price list/structure of the custom IHC services? For example, to test 200 tissue slides and get 200 images, how much does it cost? Thank you very much in advance, Have a nice weekend, Best Regards, Karen k...@prosci-inc.com

[Histonet] Rabbit antibody on rabbit tissue

Hi All, I am going to apply one rabbit polyclonal antibody on the rabbit tissue using IF. Is there anybody do it before? I heard of mouse on mouse IHC-DAB method, but not sure if it's possible in IF. Your kind response is very appreciated, Thanks, Karen _

[Histonet] control background

Hi, I am doing multilabeling immunofluorescence now and try to get rid of the background staining around tissue. It might be due to the fluochrome attached to the glass around the tissue. Is there anyway to decrease or avoid this kind of staining? Thank you, Best, Karen

[Histonet] Paraffin tissue slide quality control

Hello, I am wondering whether anybody having any experience of running the Paraffin tissue slide quality control in order to provide good tissue section? Any important factors? How to avoid wrinkles and folds in the tissue sections? We do get problems on this. Thanks in advance, Best, Karen

[Histonet] RE: Eliminating the edge effect in IHC/IF

IF To: histonet@lists.utsouthwestern.edu, Karen Cai Usually that is the result of incomplete fixation. Check your fixation protocol. Ren� J.. --- On Mon, 1/11/10, Karen Cai wrote: From: Karen Cai Subject: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu

RE: Re: [Histonet] Eliminating the edge effect in IHC/IF

Hi, Thank you very much for your input. Could you describe more with the floating section method? I never did it before. Best, Karen -Original Message- From: TF [mailto:ti...@foxmail.com] Sent: Wednesday, January 13, 2010 6:11 PM To: Rene J Buesa; histonet; Karen Cai Subject: Re: Re

[Histonet] Eliminating the edge effect in IHC/IF

? Your kind help is greatly appreciated, Thanks in advance, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax <http://www.prosci-inc.com> www.prosci-inc.com ___ Histonet mailing list Hi

[Histonet] flattening tissue surface

Hi, I need help to prepare some paraffin tissue slides for confocal microscope evaluation. We need the tissue surface very flattened, no wrinkle in order that the microscope can focus the whole section evenly. Anyone has suggestions? Thanks in advance, Best, Karen Karen Cai Research

[Histonet] Need help on Streptavidin-HRP conjugate for IHC

reply, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax <http://www.prosci-inc.com> www.prosci-inc.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.