Re: [Histonet] Histonet Digest, Vol 140, Issue 5

Alkaline Phosphatse bubbles Hi Tim, Use Dako ultramount. The bubbles are caused by the naphthyl breaking down and releasing CO2 under the coverslip. Apply small amount of the ultramount to cover the muscle tissue and bake in 65 degree oven of 15 to 20 minutes. If you bake it too long the myofibers

[Histonet] Fluorescent method FFPE

All, I need a reliable fluorescent method for FFPE tissue using mouse monoclonal antibodies. The secondary will need an emission of 488 and 594. I currently use an HRP method with DAB and would like to visualize with fluorescent method. Will only need single labeling technique. The following isoty

[Histonet] Aqueous mounting media

All, Can anyone recommend a quality aqueous mounting media? I was using Aquatex from EMD chemicals but it's been discontinued, tried Aqua-Mount from Thermo but show air bubbles a few days after coverslipping. Thanks, Kevin Bennett HT ___ His

[Histonet] succinate cytochrome c reductase

Does anyone know of a protocol for the above stain or were I can find one. Need for neuromuscular histology... Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet