Happy to second that opinion, that was probably the single most valuable
training and reference text in my career as a research neurobiologist (also
hunt down his archived Histonet posts on formaldehyde fixation, essential
reading for lab pros). Others apparently agreed, as I had to replace a co
Make sure sodium azide (antimicrobial preservative) is not used in any of your
IHC reagents--it inhibits peroxidase.
Mike
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Message: 3
Date: Fri, 1 May 2020 08:00:49 -0700
From: Kristy Castillo
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC DAB
Message-ID:
Conte
Tyrone,
I've had better luck using acetate buffer with metal-enhanced DAB. There's
less of a precipitation problem. You can also dilute the reaction solution and
the reaction product will develop more slowly, or just decrease the peroxide
concentration. You also have some control over the col
