Hi Ali,
Although I do not ever perform endothelial immunohistochemistry in our
laboratory, I know it is common to use an antibody against CD31 to selectively
stain endothelial cells and this may work well in pituitary tissue.
Hope that helps,
Kristen
Ali Nasr Esfahani alina...@student.liu.se
Shirley and Amy and others responding to this thread: thanks for your posts.
I am not in the histotechnology field - I'm actually a graduate student at
Loyola who does her own processing, cutting, and staining of bone tissue - so I
really appreciate every so-called dumb question, no matter how
I section EDTA-decalcified mouse tibias frequently, and I find it helps if the
paraffin is very very cold during sectioning - if the tissue starts shredding
again, I press a large piece of ice to the block for a minute to cool it down
without having to remove the block from the microtome. It
Hi,
I am processing rat infraspinatus tendons for Masson Trichrome staining. I've
had trouble with routine processing protocols and read that adding 4% phenol to
the 95% alcohol solutions may help soften the tissue to make it easier to cut.
Has anyone ever tried this, or have other