Re: [Histonet] Endothelial histochemistry

2010-10-22 Thread Kristen Lauing
Hi Ali, Although I do not ever perform endothelial immunohistochemistry in our laboratory, I know it is common to use an antibody against CD31 to selectively stain endothelial cells and this may work well in pituitary tissue. Hope that helps, Kristen Ali Nasr Esfahani alina...@student.liu.se

Re: [Histonet] RE: Questions

2010-09-20 Thread Kristen Lauing
Shirley and Amy and others responding to this thread: thanks for your posts. I am not in the histotechnology field - I'm actually a graduate student at Loyola who does her own processing, cutting, and staining of bone tissue - so I really appreciate every so-called dumb question, no matter how

Re: [Histonet] Trouble shooting decalcified bone sections (paraffin embedded)

2010-09-13 Thread Kristen Lauing
I section EDTA-decalcified mouse tibias frequently, and I find it helps if the paraffin is very very cold during sectioning - if the tissue starts shredding again, I press a large piece of ice to the block for a minute to cool it down without having to remove the block from the microtome. It

[Histonet] Rat tendon processing

2010-09-07 Thread Kristen Lauing
Hi, I am processing rat infraspinatus tendons for Masson Trichrome staining. I've had trouble with routine processing protocols and read that adding 4% phenol to the 95% alcohol solutions may help soften the tissue to make it easier to cut. Has anyone ever tried this, or have other