Hi, We fix 'Category-A' agent infected murine lungs in 10% buffered formalin for 7 days in ABSL-3. We also confirm tissue sterility by plating the tissue homogenates on suitable media before taking the samples out of ABSL-3 facility for sectioning. In your case 7 days should be enough, but it is always better to confirm the sterility of tissue.
Thanks, Devender Devender Kumar, D.V.M., Ph.D. Postdoctorate fellow, Center for Immunology & Microbial Disease, Albany Medical College, 47 New Scotland Avenue, MC 151 Albany, NY 12208 518.262.6220(LAB) -- -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, April 13, 2011 3:57 PM To: histonet@lists.utsouthwestern.edu; ChristinaThurby Subject: Re: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? 5 days (96 h) is more than enough, but always handle the tissue and blocks using safety precautions. René J. --- On Wed, 4/13/11, Thurby, Christina <christina.thu...@bms.com> wrote: From: Thurby, Christina <christina.thu...@bms.com> Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: Wednesday, April 13, 2011, 2:04 PM Hi, Do anyone have references for how long should autopsy/necropsy tissue be in fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should the time be extended to 5-7 days. Thanks, Christina Thurby Bristol-Myers Squibb Company Research Scientist I 812-307-2093 (tie 625) This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
