[Histonet] Acetylcholinesterase

2016-03-10 Thread Lager, Loree via Histonet
Hi Histo techs, I hope you're all being appropriately honored on our day! I wondering if anyone is still doing the Acetylcholinesterase for Hirschsprung's in children and has a procedure they're willing to share? We have a pathologist requesting it for a research project, and while we used to

[Histonet] IHC/BAL slides

2016-01-04 Thread Lager, Loree via Histonet
Hello All, Our pathologist is requesting a couple of IHC stains on air dried BAL slides, for interest. We currently only run IHC's on paraffin and frozen tissue. If anyone has protocols for CD3 and CD56, including any fixation, using the Benchmark Ultra and Ventana iView DAB detection kit,

[Histonet] Pas Digestion

2015-11-03 Thread Lager, Loree via Histonet
Hello, This is my first time using the histonet, as our veteran user retired. We've been having an intermittent problem with incomplete glycogen digestion on PASD. Our digestion solution is 0.5% α-Amylase, Type VI-B from porcine pancreas (Sigma), which we freeze in small aliquots and thaw