Why, thank you so much, Pam!
High winds/heavy rain due to hit Buffalo area starting around 5PM.
Regards,
Merced
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Barker
Sent: Monday, October 29, 2012
There are 3 reasons why it is not immediately obvious to people that one of the
links at the bottom of Histonet email helps you unsubscribe:
1. In this day and age of internet and email hypertext clutter people simply do
not have the time nor the desire to click and explore every link they come
We use Cardiac Troponin I or Cardiac Troponin T all the time (pigs). These are
also in mice.
Regards,
Merced
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos Brooks
[amosbro...@gmail.com]
It should not interfere. I use Sudan Black a lot. What concentration do you use?
Regards,
Merced
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carmen Maria Garcia
Pascual
Paraffin does indeed give a lot of autofluorescence. We use a solution of 0.3%
Sudan Black in 70% Ethanol for 10 minutes on the tissues to help with that
after the staining. It doesn't mask the autofluorescence completely but reduces
it enough to give us good contrast of signal against the
Interesting. I've never had an issue.
Regards,
Merced
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea Hooper
Sent: Thursday, January 12, 2012 10:04 PM
To: gayle callis
Cc: Histonet;
Anyone know a buffer or method (not using heat) to strip antibodies off cells
used in an immunocytostaining procedure?
The cells are in a multi-well tissue culture dish and have only been labeled
with primary (unconjugated) antibody.
Thanks.
Merced M Leiker
Cardiovascular Medicine
Biomedical
I'll seal the coverslip around all the edges and let it dry in the hood for up
to half an hour (because of the smell). I use a clear color that doesn't
fluoresce under the miscroscope and also allows the slide to be stored at low
temp (-20) without the nail polish seal cracking. Also be sure
Do you have access to a confocal microscope or one that does Z-stack imaging?
You can find out the thickness of your tissue sections that way (after you've
mounted them on a slide of course).
Regards,
Merced
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Thanks for forwarding these links. I found the comic strip and Anatech article
fascinating (in their own rights).
Regards,
Merced
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Ray
Sent: Monday,
I would definitely think it was cause components of the chamber as well as the
chamber itself to crack.
Liquid nitrogen is MUCH MUCH colder than any temperature your chamber would be,
even at its coldest. The temp difference would be too extreme.
Just my opinion having a bit of experience
96 degree water bath for half an hour in either citrate buffer (pH 6) or
Tris-HCl (pH 9), depending on which gives better retrieval for that antibody.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
I agree... I've used CD31 to stain tumor vasculature. Some tumor vasculature
isn't even lined with endothelium; there's just blood channels. But most
should be lined.
Regards,
Merced
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
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