Hi Histonetters, I'm working on embedding bacterial cells in Technovit 8100 and am curious if folks have any recommendations for dyes that can be used as a marker. I first embed my cells in a small agarose plug, then proceed to infiltrate and embed in Technovit 8100. Its possible to see the little agarose plug and flecks of cell material but can be tricky!
Does anyone have suggestions for a good dye or marker that I could mix into the agarose so I can better see when trimming and sectioning the block? The only caveat is that it must not be autofluorescent or interfere with *in situ* hybridization we do downstream on these sections. Thanks in advance! Lizzy -- "The obvious goal of any bacterium is to become bacteria." Lizzy Wilbanks Agouron Postdoctoral Fellow, Orphan Lab Division of Geological and Planetary Sciences California Institute of Technology, MC 100-23 Pasadena, CA 91125 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet