[Histonet] Xylene substitute

2019-01-03 Thread Logan, Shannon via Histonet
Hi Heather, We have been using Formula 83 by CBG Biotech for over 7 years now with no problems, we even recycle it. Shannon H. Logan B.S., HTL (ASCP) Pathology Department Bellin Health Memorial Hospital 744 South Webster Avenue Green Bay, WI 54305-3400 920-433-3653 X3727 __

Re: [Histonet] osmium tetroxide

2018-08-29 Thread Logan, Shannon via Histonet
Osmium Tetroxide is highly toxic. Storage is an issue and working with it, you must be under a hood with eye protection. I think most (if not all) labs have banned this substance? If you do not “have” to work with this material, I would recommend avoiding it. Just my opinion, thanks… Shannon H

Re: [Histonet] Special stains and hoods

2018-08-22 Thread Logan, Shannon via Histonet
That is certainly an odd request… I have never performed any special stains under a hood any place I have ever worked. Always done on a counter top near a sink… Is someone in your department extremely sensitive to odors? Or have asthma? We have a tech who experiences attacks if there are certain ch

Re: [Histonet] Problem with breast cancer TMA - core are mooving

2017-12-12 Thread Logan, Shannon via Histonet
Hello Liliane, I would suggest longer time in 60 degree oven before H&E staining… perhaps 60 min. Also, if you are not using “plus” or coated slides , I would try using them. Good luck, Shannon H. Logan B.S., HTL (ASCP) Pathology Department Bellin Health Memorial Hospital 744 South Webster Ave

Re: [Histonet] Mammary Tissue Processing & Sectioning Troubles

2017-09-27 Thread Logan, Shannon via Histonet
Hi Andrea, Tissue that is not fixed properly will be mushy and difficult to section, just as you described. Was there a reason NOT to send it in NBF for storage? I think the main problem is with the storage in 70%. Or there is a small possibility the 14 hr schedule did not remove all of the wat

Re: [Histonet] A Question About Paraffin Times

2017-05-16 Thread Logan, Shannon via Histonet
Hello Paula, So why must the cassettes be removed on Saturday morning if you aren't embedding until Monday morning? Don't you have a "delay start" feature on your processor? Neither option A or B seem like a good thing for the tissue! We time our processor to finish at 5 AM Monday when the first

Re: [Histonet] block retention

2016-11-08 Thread Logan, Shannon via Histonet
We keep our blocks and slides for 10 years. Shannon H. Logan HTL (ASCP) Pathology Dept. Bellin Health 744 S Webster Ave Green Bay, WI 54305 920-433-3653 -Original Message- From: Teresa W. Gainey via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, November 08, 201

Re: [Histonet] Static electricity Tip

2016-06-01 Thread Logan, Shannon via Histonet
Glad to see this postI also use the dryer balls too (two tennis balls) and it helps greatly!! No more static and cutting is much more "tame". Not to mention that it is healthier because dryer sheets are full of chemicals and toxins. Have a good one... Shannon H. Logan B.S. HTL (ASCP) Pa

[Histonet] FW: FW: Freeze Spray Ban continued

2016-03-01 Thread Logan, Shannon via Histonet
Subject: RE: [Histonet] FW: Freeze Spray Ban continued http://www.fridgehub.com/News/epa-fact-sheet-and-timelines-for-proposed-hfc-bans After reading this article, seems to affect only cars, refrigerators, and certain air conditioning units Shannon H. Logan B.S. HTL (ASCP) Pathology D