[Histonet] centrifuge

2013-04-12 Thread Margaret Horne
Hello, I am posting this for a group of veterinary students that volunteer for the local Greyhound Rescue chapter. Just a faint hope. They are looking for a donated centrifuge (benchtop, 5 ml tubes). It is to test the blood samples of rescued dogs to check for parasites before shipping the dogs

[Histonet] gently used Tissue Processor

2013-04-09 Thread Margaret Horne
Hi , Is anyone interested in buying a Leica ASP 300 S Tissue Processor. Bought in 2008. used for 1 year. Margaret You can contact me by email : mho...@upei.ca or phone 902-566-0930 ___

histonet@lists.utsouthwestern.edu

2013-03-27 Thread Margaret Horne
u use will determine if it is ok to cover slip from IsOH. We just had a grad student compare Hot IsOH vs mineral oil vs Hot detergent as xylene free alternatives. I'll send you our final protocol if you like. Margaret Horne AVC, UPEI, "Gerard Spoelstra" 2

[Histonet] cost of histology

2012-12-04 Thread Margaret Horne
Hello Everyone, I am trying to figure out the cost of materials alone for Embedding, and a simple H&E. We have a multi-user facility where the Researchers do the work, actually their techs ;-) , but I would like to have them replace the supplies used. Slides and blades are easy as they can b

Re: [Histonet] mouse testis in Bouins- more info

2012-09-14 Thread Margaret Horne
EtOH better for long term ( months to years) storage? Thanks everyone, Margaret >>> "Margaret Horne" 14/09/2012 10:06 AM >>> Hello Everyone, I am asking this for a friend. How long can mouse testis be kept in Bouins without distortion of cell morph

[Histonet] mouse testis in Bouins

2012-09-14 Thread Margaret Horne
Hello Everyone, I am asking this for a friend. How long can mouse testis be kept in Bouins without distortion of cell morphology? Days? weeks? months? years? I noticed in the Archives that many people fix in Bouins , rinse, then store in 70% EtOH. This is preferable I assume. Again, how long i

[Histonet] plants

2012-05-15 Thread Margaret Horne
I had to remove my plants from a lab because people were doing something ( RNAse ? ) that could be contaminated by air borne plant material. However that was the only time. Any other labs were not worried about it. I even repot plants in my present lab. The questions is " What would happen if p

[Histonet] Re: Pinning Specimen

2012-04-20 Thread Margaret Horne
Just another idea : I have used dental wax. Comes in a package of about 20 sheets pf pink wax, each 7.5 cm x 14.5 cm x 0.2 cm. It's a little bit more pliable than the waste paraffin, but not as cheap :-) I cut it in a long rectangle so that it sticks out of the formalin with the tissue at the bo