Re: [Histonet] Coverslipper

Happy Thursday, We would like to sell our slightly used but in excellent condition Dako Coverslipper which was purchased in 2010 (purchase price was $ 25K). The instrument can handle up to 600 slides per hour making it one of the fastest on the market. In addition to the flexibility of the

[Histonet] X-gal staining

Hello histonetters, I have request from a scientist to perform X-gal staining on the formalin fixed paraffin-embedded kidney. I was wondering if there is any possibility to do this staining on FFPE tissue as well as if there any possibility to perform a peroxidase DAB staining for the X-gal?

[Histonet] job search in Chicago area

Hello histonetters, Please share this email with your colleagues. I am in a job searching process in Chicago area to be close to my family as my appointment in West Virginia University Research Corporation, Morgantown, WV is coming to an end since my long-standing supervisor is closing her

Re: [Histonet] Tissue scrolling

I do the same way! Good luck! Naira From: Colleen Forster Sent: Sunday, September 8, 2019 12:43 PM To: John Garratt Cc: Erin McCarthy ; histonet-request Subject: Re: [Histonet] Tissue scrolling We cut alot if these. This is the protocol: 1. The microtome,

Re: [Histonet] IHC mouse optomization

Biocare Medical has pure ready to use secondary.. Naira -Original Message- From: Blanca Lopez Sent: Wednesday, June 12, 2019 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC mouse optomization Morning, I need a good advice in optimizing Mouse antibodies for

Re: [Histonet] Coverslipper

Happy Thursday, We would like to sell our slightly used but in excellent condition Dako Coverslipper which was purchased in 2010 just for $10K (purchase price was $ 25K). The instrument can handle up to 600 slides per hour making it one of the fastest on the market. In addition to the

Re: [Histonet] Coverslipper

Happy Friday, We would like to sell our slightly used but in excellent condition Dako Coverslipper which was purchased in 2010 $10K (purchase price was $ 25K). The instrument can handle up to 600 slides per hour making it one of the fastest on the market. In addition to the flexibility of the

Re: [Histonet] Coverslipper

Happy Thursday, We would like to sell our slightly used but in excellent condition Dako Coverslipper which was purchased in 2010 $10K (purchase price was $ 25K). The instrument can handle up to 600 slides per hour making it one of the fastest on the market. In addition to the flexibility of

[Histonet] Polyclonal Rabbit Anti-Cytokeratin

Hello, I was asked to do IHC using the Polyclonal Rabbit Anti-Cytokeratin, Wide Spectrum Screening, Polyclonal, Unconjugated, Ig fraction, (Z062201-2). Ca anyone help me with AR to use and concentration to start. Thanks in advance, Naira ___

Re: [Histonet] Coverslipper

Happy Thursday, We would like to sell our slightly used but in excellent condition Dako Coverslipper which was purchased in 2010 $10K (purchase price was $ 25K). The instrument can handle up to 600 slides per hour making it one of the fastest on the market. In addition to the flexibility of

[Histonet] coverslipper CR10030

Dear histinetters: We can offer our slightly used DAKO coverslipper CR10030 for purchase. Let us know if you are interested. Thanks, Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is

[Histonet] PD-L1 22c3 for IHC (paraffin)

Dear Friends, Can any of you share with me a full detailed protocol (time and dilutions) after deparaffinization for the PD-L1 22c3 for IHC (paraffin) to use on DAKO (not Link 48) autostainer?

[Histonet] PD-L1 and TGFb for FFPE human tissue

Good morning everyone, I was wondering if anyone can give me an advise regarding the best Abs for the PD-L1 and TGFb for FFPE human tissue (except DAKO Abs, please). Thanks in advance, Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0)

[Histonet] Immunoperoxidase Protocol on Cytospin

Dear histonetters: I never done ICC. A scientist brought me a slide with Cytospined cells on for IHC. I am going to fix with 10%NBF (it is only what I have now) then wash with TBST. May I skip AR step? May I use my usual IHC protocol and reagents which I usually use for FFPE tissue: blocks

Re: [Histonet] Recycled reagents in VIP processor

; When people say they are saving money by recycling reagents I always > wonder if they are including tech time (to run the still) in their > calculations. > > Sincerely, > > Jay A. Lundgren, M.S., HTL (ASCP) > >

Re: [Histonet] Recycled reagents in VIP processor

WOULD LIKE TO KNOW AS WELL - IT IS WASTE OF MONEY Thanks, Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named

[Histonet] rat vomeronasal organ (VNO) fix, decal, proc. and paraffin embedding protocol

Dear Histonet Colleagues, I hope you had very nice and warm holiday season . We are interested in a routinely used protocol to decal (if needed), process and paraffin embed the rat vomeronasal organ (VNO). It would be greatly appreciated if anyone would be willing to share a protocol for