Hello All,
We section fixed frozen brain that we mount on the microtome using either 30%
sucrose or OCT and we keep the temperature down by surrounding the tissue with
crushed dry ice constantly (every 10 minutes).
One of the problems I have had, and also the person who trained me also had, is
Hello Histonet community,
I recently froze a primate brain using isopentane immerse in a bath of 100%
ethanol and chunks of dry ice. Two out of the 3 blocks froze fine but the third
one shows a very bad convex surface, although to be fair, the defective block
was embedded in gelatin while the
