[Histonet] HSV I/II Control

Awhile ago I had posted looking for anyone that had any in house HSV controls. A gentleman had reached out to me; however, I never did receive the controls that he said he was sending me. I am trying to reach him. I am also looking for anyone else that may also have HSV controls. We are in

[Histonet] HSV 1/11

Does anyone have any positive control blocks for HSV? Or for those that are buying their control material any suggestions? Thank you in advance! Sara J. McCabe, HT(ASCP)CM Histology Supervisor Penn Highlands DuBois 100 Hospital Avenue DuBois, PA 15801 814-375-7514 Telephone-Office 814-375-3264

[Histonet] Cytology fixative

Wondering what everyone is using for their "wet" respiratory cytology specimens(bronch specimens, BAL, brushings etc) Currently we have our microbiology department use mucolytic agent. We also use cyto rich red for our brushing specimens. We are having a "drying out" effect and are wondering

[Histonet] CMV controls

Hello all, I am looking for positive CMV controls for use for IHC. If anyone has any that they would be willing to share or trade for other tissue control material please message me! Also, if not, where are you buying your CMV control slides/material from? Thank you in advance! Sara J.

[Histonet] Cytology Staining

I have a question for those of you that also may perform cytology specimens at your facility. How do you prevent carryover from one case to another? For example, we had obvious carryover from a pleural fluid onto an FNA of a thyroid case. This has never happened here (to our knowledge). Has

[Histonet] RE: p16 on Bond

Richard, We currently do not dilute the p16 antibody. We run it on the Bond using ER1 for 20 minutes. Sara McCabe, HT (ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named

[Histonet] RE: Stain for Hp

Routinely we do a Diff Quik stain on all stomach biopies. If the Pathologist requests we do an IHC using Biocare's antibody on the Leica Bond. Sara McCabe, HT (ASCP) DuBois Regional Medical Center ___ Histonet mailing list

[Histonet] RE: Histonet Digest, Vol 104, Issue 28

Hi Donna, We use Novocastra/Leica Cyclin D1 (CYCLIND1-GM-L-CE) 1:50. We use a mantle cell lymphoma as control. Sara McCabe, HT (ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the

[Histonet] IHC on Bones

Hello Histonet, Wondering what those of you that do IHC on bones do to ensure that the tissue remains on the slide during processing? Thanks, Sara McCabe HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended

[Histonet] PMS2 Mismatch Repair

Hello Histonetters, Wondering if anyone is using PMS2 Mismatch Repair Enzyme from Novocastra/Leica. Have tried varied dilutions and retrievals without any luck. Any suggestions would be great! Thanks! Sara McCabe, BS, HT (ASCP) DuBois Regional Medical Center This email and any attached files

[Histonet] CMV IHC

Happy Friday Histonetters! Anyone performing CMV by IHC? If so what are you using for a protocol? We ordered CMV from Cell Marque but they give no suggestions for a protocol. We are using the Leica Bond for our IHCs. Any input is greatly appreciated! Thanks! Sara McCabe, HT (ASCP) DuBois

[Histonet] RE: controls on patient slide

We always put our patient and control tissue on the same slide. We have many controls already cut and then we place the patient tissue below. If we are using blanks that were previously cut then we cut the control and place that below the patient tissue. We chose to not invest in the

[Histonet] RE: p16 on the Bond

Hi Nancy, We too use p16 on the Bond Max and Bond III using Leica ER 1 for 20 min. It is working great for us! Sara McCabe, HT (ASCP) DuBois Regional Medical Center From: histonet-boun...@lists.utsouthwestern.edu