[Histonet] FLAG control slides for IHC

Does anyone know of a commercially available source for FLAG (DYKK) IHC control slides? Thank you. Michele ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IHC control slides for EBNA

Does anyone know of a source of IHC control slides for EBV Nuclear Antigen? Thank you. Michele ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FJC controls

Does anyone have any good Fluoro Jade C controls that they would be willing to sell or possibly trade for some other control blocks? Thanks, Michele ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] ORO staining in pre- vs. post-fixed tissue

Does anyone know whether there is a difference, quantitatively, in Oil red O fat staining of tissue fixed in NBF prior to embedding in OCT vs. fresh frozen tissue embedded in OCT? I know that sections from fresh frozen tissues must be post-fixed in formalin before staining with Oil Red O. But

RE: [Histonet] Congo Red control slides

American Mastertech, Cat. #CSA0425P -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mesruh turkekul Sent: Tuesday, August 30, 2011 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Congo Red

RE: [Histonet] Where Can I Buy Pneumo Control Slides

American MasterTech sells them: http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStylesite m=CSP015P -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of wanda.sm...@hcahealthcare.com Sent:

[Histonet] pan B-cell marker for frozen mouse tissue

I am trying to find the most appropriate pan B-cell marker for IHC on frozen mouse tissue. I know that CD45R/B220 used to be the most commonly used, but recent publications suggest PAX5 or CD19 as more restricted to the B-cell lineage. Does anyone have any other suggestions, or are these generally

[Histonet] BrdU on rat tissue

Can anyone recommend a good commercially available kit for BrdU on rat tissue? Thanks in advance for any suggestions. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] TNF alpha antibody

Can anyone recommend a good TNF-alpha antibody for frozen mouse tissue? Any suggestions are greatly appreciated! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] CD31 on rat frozen tissue

I realize that CD31 staining has been discussed extensively in the past on histonet, but typically the conversation revolves around which fixatives to use for paraffin sections or the inability to find a good CD31 antibody for mouse tissue. I am simply looking for a CD31 antibody that works well

[Histonet] osteopontin

Can anyone recommend an osteopontin antibody for inflammation that works in FFPE mouse tissue, in particular mouse paws? This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you

[Histonet] removing trigeminal nerve and ganglion

Does anyone have experience/a good method for removing trigeminal nerve and ganglion from rat intact? Any advice would be helpful. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure.

[Histonet] osteopontin antibody

Can anyone recommend an osteopontin antibody that works in FFPE mouse tissue? This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note

[Histonet] removing coverslips

Hello. Can anyone tell me how I might remove coverglass from slides containing GMA sections mounted with UV mount (PolySciences)? Thank you. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from

RE: [Histonet] Stat-Q Staining Kit

We have also had great results with it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle Sent: Thursday, June 04, 2009 2:29 PM To: histonet Subject: [Histonet] Stat-Q Staining Kit Does

[Histonet] MetAP-2

Has anyone successfully used Santa Cruz's MetAP-2 for IHC in FFPE mouse and rat sections? I am starting for scratch and am trying to find info on dilution range and antigen retrieval methods. The spec sheet only lists recommendations for applications other than IHC. Any info is greatly

RE: [Histonet] Dako

Innovex is a wonderful company that offers good products at an extremely reasonable cost and provides exceptional tech support. No, I don't work for them...I've just had great experiences. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] aqueous mounting media

Can anyone recommend a good aqueous mounting media for coverslipping Oil Red O stained slides? We are currently using Dako's Faramount media, but have been having problems with excessive air bubbles; especially, it seems, in the tissues containing a lot of fat. I recently heard that Dako's

[Histonet] job posting

Seventh Wave Laboratories, located 30 miles west of downtown St. Louis, MO is currently seeking a Histotechnologist whose primary functions will be microtomy and staining of histological specimens. Other duties may include tissue trimming, processing, and embedding. Interested candidates may

[Histonet] at a troubleshooting loss

A couple questions for those who are troubleshooting savvy: Other than inadequate fixation, water being introduced during processing, or too much heat, what can cause fuzzy, uneven hematoxylin staining with poor nuclear detail? And secondly, aside from formalin, are there any other common agents

RE: [Histonet] Formalin and Xylene Monitoring Badges

Morphix Technologies www.morphtec.com 757-431-2260 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Kathleen Boozer Sent: Friday, October 31, 2008 11:22 AM To: histonet@lists.utsouthwestern.edu; Sandra Cheasty Subject: Re: [Histonet] Formalin and Xylene

[Histonet] cryostat SOP/fibrin and plasmin antibodies

Thanks again for all responses to my cryostat SOP question. I guess it's time now to write the dang thing. While I'm on here, can anyone recommend a vendor for fibrin and plasmin (plasminogen) antibodies that are reactive in mouse tissue and possibly share a working dilution for either of

[Histonet] myoglobin antibody

Hello, I'm looking for a myoglobin antibody that reacts in rat tissue and works in FFPE tissue. Can anyone recommend a vendor? Thanks for any info. ~Michele This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential

[Histonet] Okajima's stain

This is probably a really obvious question, but when doing Okajima's stain for hemoglobin, is a blood smear an adequate control? Thanks for any advice. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or

[Histonet] immuno diluent vs. buffer question

I have a question for those doing immunos by hand. I typically dilute my primary, secondary and tertiary antibodies with manufacturer supplied diluent. Recently, I was running a test to determine the source of a mysterious precipitate that had begun to appear on my slides (It has since then