Re: [Histonet] IHC help

2024-08-01 Thread Michelle Bell via Histonet
If you’re doing online retrieval/deparaffinization, try deparafinizing offline. I found the paraffin we loved had was higher in polymers and resulted in the tissue being prone to lifting. As soon as we started deparafinizing BEFORE putting the slides on the instrument, we had greater (not perf

Re: [Histonet] microwave processing

2024-02-15 Thread Michelle Bell via Histonet
From the vendor perspective, we have definitely seen an increase in implementation of our processors. Going to the xylene-free, isopropanol clearing approach has lead to converts because the process is more gentle (xylene can harden dense tissues and over clear small specimens). Before coming

Re: [Histonet] Room temperature optimal for Histology Lab

2023-05-24 Thread Michelle Bell via Histonet
Unfortunately, there are no “best practices” for room conditions that I can find. The only real standard is to follow the manufacturer’s guidelines for reagents AND equipment. There is a reference to ideal ranges on the FDA website, but it’s specifically refers to clinical labs. I look forward

Re: [Histonet] baking after coverslipping

2023-05-22 Thread Michelle Bell via Histonet
We baked our slides in our IHC oven on metal trays (for cytology) overnight so we could file them. 15 minutes wouldn’t have worked, but maybe they are using a hotter oven. As for the air pockets, the baking won’t solve that. This usually (but not always) happens when the ratio of mounting med

Re: [Histonet] Who sells that block deparaffinizer dohickey that everyone has in their lab.

2023-03-01 Thread Michelle Bell via Histonet
I don’t know the brand, but they are called paratrimmers. Multiple vendors carry they such as Newcomer or Mercedes. My techs loved them! From: Jay Lundgren via Histonet Sent: Wednesday, March 1, 2023 6:04:37 PM To: histonet Subject: [Histonet] Who sells that bl

Re: [Histonet] What is considered Grossing according to CLIA and CAP? Definition?

2023-02-27 Thread Michelle Bell via Histonet
This is so hard to unpack everything that could be wrong with this situation. The weights the lab assistants take would lead me to the conclusion that this would be part of grossing, because it is reportable. It’s such a slippery slope when you start delegating tasks associated with high compl

Re: [Histonet] pathologist competency

2022-09-26 Thread Michelle Bell via Histonet
We included random reviews along with any discrepancies and how they were handled. If you look through the CLIA brochure for competency assessments, it defines all of the required elements. You need to cover each of the points mentioned somehow. I included the link :) https://www.cms.gov/regul