[Histonet] Toluidine Blue Dilution

Hey Netters, Does anyone have a procedure for diluting Toluidine Blue from powder or 1% aqueaous with 10% NBF. I have always eyeballed the measurement as I was taught. However, as a new sup, I need to make sure we have an SOP in our manual. Thanks All, Natacha Mitchell, BS, HTL (ASCP) Histolo

[Histonet] IHC Staining Platforms Pros/Cons

Hello fellow Histonetters, I was wondering if anyone would be willing to share their input regarding the differences, pros/cons and experiences with the Leica Bond III, Ultra Benchmark and Dako Omnis IHC staining platforms. Currently, we have two Ultra Benchmark strainers and use all prediluted

[Histonet] Protocols with unstained slides

Hello Fellow Histonetters, We are currently in the process of revising our protocols to reduce the amount of unstained slides cut. Currently, we cut unstains for prostate bx's, (2 H/E, 1 UNS), liver bx's (2 H/E's, 6 UNS), bone marrows (2 H/E's, 6 UNS) and cytology blocks (2 H/E, 8 UNS). I do re

[Histonet] Embedding Centers

My fellow histonetters, Thank you all so much for the prompt response. All the posts/emails were very helpful and I must say this site is a priceless source of knowledge, wisdom and resource!!! Natacha Mitchell CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediate

[Histonet] Embedding Centers

Hello fellow histonetters, Can you all provide input on experience with the Leica EG 1150 embedding center, Thermo HistoStar and Tissue-Tek TEC 5. I have worked with all but the Leica model. Thanks in advance!! Natacha Mitchell CONFIDENTIALITY NOTICE: If you have received this e-mail in error