[Histonet] RE: Slide Consults

2014-03-19 Thread Mitchell Jean A
Amber: I find this EXTREMELY irritating when an outside institution either writes their accession number on the label or puts another label over the top of my hospitals labels. It is not rude to ask other hospitals to not do this; that doesn't mean they won't do it. I will forward you my slid

[Histonet] RE: Leasing an Electron Microscope

2014-02-26 Thread Mitchell Jean A
James: Depending upon the amount of EM work your have - another option is to "lease" scoping time from another facility in your area that maintains an EM scope. My lab pays for scoping time on a per hour basis. You of course have to work with the owner of the scope for availability but it wo

[Histonet] 2014 Tri-State Histology Symposium

2014-02-18 Thread Mitchell Jean A
Registration is open for the Minnesota, Wisconsin and Iowa 2014 Tri-State Spring Histology Symposium that will be held at the DoubleTree Hotel Rochester, Minnesota April 30-May 2. There is an outstanding program of seminars and workshops that begin Wednesday afternoon April 30th and conclude

RE: [Histonet] Unregistered HT

2013-09-11 Thread Mitchell Jean A
Even if a facility does not require that techs be HT registered - the technicians themselves need go that extra step, take the initiative themselves and become HT certified. If a portion of histologists don't recognize or care about the merit of certification how can we progress our profession

RE: [Histonet] Oil red O

2013-09-09 Thread Mitchell Jean A
I believe the question is: what does the "O" in Oil Red O stand for? Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto

RE: [Histonet] Extra Money

2013-05-30 Thread Mitchell Jean A
To clarify: Winning NSH poster presentations in both Clinical and VIR categories each receive $250.00 - but NSH does not reimburse any expenses for poster presentation. Hope no one got their hopes up on this Jean Mitchell -Original Message- From: histonet-boun...@lists.utsouthwes

RE: [Histonet] Isopentane storage-the polling is open

2013-04-16 Thread Mitchell Jean A
We maintain an approximately 1 liter supply of isopentane and store all isopentane (open/unopened) in an explosion proof freezer. Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -Original Message--

[Histonet] Thioflavine S for Amyloid

2013-04-12 Thread Mitchell Jean A
Would appreciated some feedback/input from labs using Thioflavine S staining protocol for amyloid screening. Any advantages/disadvantages to this procedure vs Congo Red? Thanks much!! Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland A

[Histonet] Tri-State Histology Spring Symposium

2013-04-08 Thread Mitchell Jean A
Reminder: Registration is in the last weeks for the Iowa, Wisconsin and Minnesota Tri-State Spring Histology Symposium that will be held at the Hotel Julien, Dubuque, Iowa April 24-26th. There is an outstanding program of seminars and workshops that begin Wednesday afternoon April 24th and con

[Histonet] RE: minus 80 freezer safe time in power outage

2013-03-29 Thread Mitchell Jean A
Tim: speaking from experience (unfortunately) and the failure of a -80/-70 freezer. As you would imagine once the power goes out or the freezer malfunctions - the freezer temperature rises very quickly. I would estimate, and without opening the door, at the most you have 1-2 hours before you

RE: [Histonet] Safranin O staining

2013-02-18 Thread Mitchell Jean A
I was just thinking about Russ Allison the other day. He is certainly missed. Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -Original Message- From: histonet-boun...@lists.utsouthwestern.e

[Histonet] Alpha-Dystroglycan IHC

2012-10-29 Thread Mitchell Jean A
Wanted to know what labs performing neuromuscular IHC are running Alpha-Dystroglycan. Would appreciate if you would share your source and if you have experienced any issues with this antibody within the past year. It has become my problem child. Jean Mitchell, University of Wisconsin Hosp

[Histonet] RE: Renal biopsies and muscle biopsies

2012-10-22 Thread Mitchell Jean A
Jim: I work in a "stand alone" neuromuscular lab and we do go directly to the OR's to retrieve muscle biopsy tissue. We offer this service for a number of reasons; we maintain excellent communication with surgery staff on muscle bx requirements; we do triage the tissue received on-site to de

RE: [Histonet] Phosphorylase method

2012-03-28 Thread Mitchell Jean A
procedure to you in a separate email. The procedure has clean results, is permanent; and can be coverslipped with regular mounting media (I use cytoseal). Jean -Original Message- From: Sharon Allen [mailto:sal...@dsmanitoba.ca] Sent: Wednesday, March 28, 2012 11:12 AM To: Mitchell Jean A Cc

RE: [Histonet] Re[2]:

2012-03-27 Thread Mitchell Jean A
How about these postings to histonet? Is everyone else getting these from Tuyen Nguyen & Diamond Ranch Music? If so - can these people/email addresses be deleted from the histonet service? A little redneck humor is one thing - but the love boutique sex shop is another. Jean Mitchell -

RE: [Histonet] Diamond Knives

2012-03-15 Thread Mitchell Jean A
Micro Star Technologies, Huntsville, Texas is also a supplier of diamond knives and a resharpening service. I have used them many times over the past 20+ years and have been satisfied with their product. Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Labor

[Histonet] 2012 Tri-State Symposium

2012-03-05 Thread Mitchell Jean A
Dear Histonetters: You are invited to join the histology societies of Wisconsin, Iowa and Minnesota as they host "An Ocean of Knowledge" at the 2012 Tri-State Symposium, May 2-4 at The Concourse Hotel in Madison, Wisconsin. For program, registration and vendor/exhibit information contact the f

[Histonet] EM Processors

2011-12-02 Thread Mitchell Jean A
Looking for input from hospitals/institutions that process tissues for electron microscopy. I'm interested in what automated (such as Lynx) or microwave processors for EM are being utilized. What if any issues you have had and what you would recommended. Thanks for any and all feedback. Jean

[Histonet] NSH - One Day VIR Forum & VIR Teleconference Series

2011-03-01 Thread Mitchell Jean A
One Day VIR Forum Saturday, March 26, 2011 < 8:30 am - 4:15 pm Hampton Inn & Suites in Raleigh, NC Sessions Include: * Digital Pathology - A New Era for Pathology presented by Curtis Adams, PhD, Sr. Product Manager, Life Sciences, Aperio * Developing, Trouble Shooting, and Optimi

RE: [Histonet] Previously Cut Slides for FS on Muscle

2011-02-14 Thread Mitchell Jean A
Did you run an H&E or any other stain on the case before you stored the slides at -80? The muscle tissue very likely was dried out and had artifact to begin with even before you stored the sections/slides in the freezer. I have stored muscle sections for days/weeks/months at -80 without any adv

[Histonet] Lysine Buffer

2010-09-22 Thread Mitchell Jean A
I use minimal quantities of lysine buffer in PLP fixative and my protocol notes a shelf life of one month for the buffer solution. Instead of making up so often has anyone frozen lysine buffer in small quantities and thawed prior to use? Jean Mitchell, BS, HT (ASCP) University of Wisconsin Hospit

[Histonet] 2010 Tri-State Symposium

2010-03-15 Thread Mitchell Jean A
Dear Histonetters: You are invited to join the histology societies of Iowa, Minnesota and Wisconsin as they host the 2010 Tri-State Symposium, April 28-30, at The West Des Moines Marriott Hotel, West Des Moines, Iowa. For program & registration information visit our websites or contact the fol

RE: [Histonet] FW: RIP Russ Allison

2009-11-12 Thread Mitchell Jean A
I had the great privilege to speak with Russ Allison at a conference in Australia several years ago. He was a funny man, he was a wild man and enjoyable to be around. His birthday was October 31st - and a Halloween has never gone by since or will go by that I won't think of him. Cheers Russ!!

[Histonet] RE: IHC free floating sections

2009-07-31 Thread Mitchell Jean A
Michael: I use 1.5mm nichrome transfer loops for IHC staining of 50um free floating sections and I move them into clean culture wells for each solution. I purchase mine from Ted Pella. It does takes some practice but the loops work quite well with minimal damage to the tissue. Jean Mitchell, BS

[Histonet] 1st Time Attendees to NSH S/C Birmingham

2009-07-16 Thread Mitchell Jean A
Will you be a 1st time attendee to the NSH Symposium/Convention in Birmingham, Alabama in October? Or have not attended an NSH S/C in at least 5 years? If so - you may be eligible for $500 to attend the symposium and be the recipient of one of nine $500 Newcomer-Newcomer Awards. The 4 requireme

[Histonet] NSH Call for Awards Applications/Nominations

2009-04-29 Thread Mitchell Jean A
Last Call for Nominations/Applications: You still have time to make the NSH 2009 Awards/Scholarship May 1st deadline! Each year, NSH recognizes the commitment and achievements of individuals in the histology profession, spotlights students and their achievements, and applauds the innovation

[Histonet] 2009 Tri-State Symposium

2009-03-10 Thread Mitchell Jean A
You are invited to join the circus and clown around with the histology societies of Wisconsin, Minnesota and Iowa as they host "Big Top Histology" the 2009 Tri-State Symposium, April 29-May 1, at The Madison Concourse Hotel, Madison, Wisconsin. The inclusive registration fee of $100 (+ state membe