Hello veterinarian histonetters,
We have *VIP5, VIP6 and Leica *ASP300.
We run cassettes with 4mm tissues sections from all organs after the
necropsy. Our species are dogs, rabbits, rats and mice. We try to keep a case
together that is why running all cassettes from one animal at the same
time.
Hello histonetters,
Could you please share your protocols for running animal tissues from full
body necropsies of mouse, rat, rabbit, pig, dog?
Thank you in advance,
Naira
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Hello,
Could you please share your processing programs to run mouse, rat, rabbit,
dog’s organs after full necropsy?
Thank you in advance for sharing your different programs,
Naira
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Interesting. I never baked my slides. Left air dry o/n on room. Next day
started with deparaffinization steps 2x30min. My most AR where in the
citrate buffer in the water bath with 92C(if I remember correctly) for 37
minutes then 12 minutes cooling at room temperature. Never had a problem.
Naira
O
Hello,
We have noticed lots of wax dots on the hallway with linoleum floors.
Sticky mats are not helping. Even when we leave our lab coat in the lab,
there is still some residue under our shoes.
What do you use in your lab?
How you remove wax from the shiny linoleum surface?
Any suggestions are
Hello,
Wam interested to know what you are using to reduce static of paraffin
sections.
Please share your experience and expertise.
Thanks in advance,
Naira
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Hello,
What type of ALCOHOL ETHYL you’re using in your histology lab.?
We are thinking to get 20-30-gal drum.
Could you please help me with that?
Your suggestion is appreciated,
Naira
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Hello!
What is the best company to buy a ready to use Modified Davidson solution?
Thanks in advance,
Naira
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Hi, I would like to learn as well!
Thanks,
Naira
On Fri, Mar 1, 2024 at 6:59 AM Frances Pearsall via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Hi histo-peeps, I would like to know any one's experience with the Epredia
> Revos Tissue Processors especially animal related tissues?
>
>
Hello,
Could you please share your best recipe for the Modified Davidson?
Thanks in advance,
Naira
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gt;
> I have this set up and it works great.
>
> Colleen Forster HT(ASCP)QIHC
>
> On Tue, Nov 7, 2023 at 3:58 PM Naira Margaryan via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
>> Hello,
>>
>> We are thinking to process the dog stifle joint
Hello,
We are thinking to process the dog stifle joints. For that reason we will
need large cassette/slides (3x2) and more importantly to find a large block
holder for the Leica Microtome.
Any advice and suggestions where to purchase all large parts would be
appreciated.
Thanks in advance,
Naira
Hello,
Animal tissue too dry after processing.
Could you please suggest the best processing protocols for large (dog,
rabbit) and small (rat, mice) animal tissues?
Thanks in advance,
Naira
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htt
Hi Tony,
Very interesting article you shared.
Hi Gudrun,
I will only add to this - run positive and negative tests for every run.
Good luck!
Naira
On Sat, Jul 1, 2023 at 4:40 PM Tony Henwood via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Hi Gudrun,
>
> This should be useful ( I wi
Hi Charles,
I always ran serial sections for negative control - so, yes, the same
species.
Good luck,
Naira
On Tue, Feb 21, 2023 at 2:58 PM Charles Riley via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> When performing IHC's for research projects is it recommended to use the
> same sp
Yes, I use it for almost a year and I love it. Only thing is that you have
to take good care of the unit.
If you have more questions I will be happy to answer,
Naira
On Fri, Jan 20, 2023 at 1:07 PM M.O. via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Hello and Happy Friday!
> I work in
Thank You so much Jay, for such detailed explanation and for permission to
use your email to address.
My sincere regards,
Naira
On Fri, Sep 9, 2022 at 5:00 PM Cooper, Brian wrote:
> Thanks for saying this Jay!! I have to say, it's been a while since we've
> had such a great response on Histonet
Thank you all who answered.
I also would like to get any research behind that - maybe a publication to
prove.
If anyone come across with scientific publications please share it with me.
Thanks in advance,
Naira
On Wed, Sep 7, 2022 at 1:23 PM Terri Braud wrote:
> Just my 2 cents. Warm molds a
Hello histo geeks,
Could you please help me with project and provide me best reasons why using
warm molds are better than cold (room temperature) as well as the opposite
why not to use room temperature molds during embedding tissues.
Reason I am asking that we were suggested to use cold to easil
Thanks everybody and have a nice day!
Naira
On Fri, Dec 18, 2020 at 3:53 PM Naira Margaryan
wrote:
> Hi,
>
> Please help me to remember what concentration of Ammonia water need for
> microtome hard paraffin blocks?
>
> Thanks in advance
> Naira
>
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Hi,
Please help me to remember what concentration of Ammonia water need for
microtome hard paraffin blocks?
Thanks in advance
Naira
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