Hi Eleanora We do trichrome on muscle but ph of solution we keep at 3.4 and first hematoxylin then trichrome for 10 minutes and then wash in water 2-3 times and then two changes of 95 percent alcohol then DCM
Naveeda On 2013-05-15, at 1:02 PM, histonet-requ...@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Lysol IC for Ventana equipmetn cleaner (Tom McNemar) > 2. Re: [IHCRG] p40 (Richard Cartun) > 3. RE: Gross Room cleaner (Sarah Dysart) > 4. RE: Re: [IHCRG] p40 (Patsy Ruegg) > 5. Bone marrow aspirate iron stain (Renz, Jason) > 6. Gomori Trichrome Staining (Eleonora Guadagnin) > 7. Re: Gross Room cleaner (Paula Sicurello) > 8. RE: Bone marrow aspirate iron stain (Tony Henwood (SCHN)) > 9. RE: Lysol IC for Ventana equipmetn cleaner (Sebree Linda A) > 10. HPV In situ hybridization protocol (FISH or CISH) (Mesru T) > 11. Mohs histology hourly rate (rook...@comcast.net) > 12. Re: Mohs histology hourly rate (Will Chappell) > 13. Re: Gomori Trichrome Staining (Massimo) > 14. Re: Mohs histology hourly rate (Jaclyn Pitts) > 15. Re: Mohs histology hourly rate (Will Chappell) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 14 May 2013 13:15:20 -0400 > From: Tom McNemar <tmcne...@lmhealth.org> > Subject: [Histonet] RE: Lysol IC for Ventana equipmetn cleaner > To: 'Amber McKenzie' <amber.mcken...@gastrodocs.net>, > "Histonet@lists.utsouthwestern.edu" > <Histonet@lists.utsouthwestern.edu> > Message-ID: > <E9A90E28259D2F4E84308C5E8EA8F7B4014717F5E8CB@lmhs-exchange> > Content-Type: text/plain; charset="us-ascii" > > We use it but we only make it up 1 gallon at a time. We use 20ml to 1 gallon > of distilled per Ventana. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcne...@lmhealth.org > www.LMHealth.org > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie > Sent: Tuesday, May 14, 2013 12:33 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Lysol IC for Ventana equipmetn cleaner > > > I used to use Amphyl to decon my Ventana equipment, but now Cardinal sells > Lysol IC in its place and I was wondering how you guys make it up...water to > cleaner ratio in the Vantana carboys. With Amphyl I did 20L dH20/200ml > Amphyl. Thanks! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the > individual and/or entity to whom it is addressed, and contains information > from Licking Memorial Health Systems which is confidential or privileged. If > you are not the intended recipient, nor authorized to receive for the > intended recipient, be aware that any disclosure, copying, distribution or > use of the contents of this e-mail and attachments is prohibited. If you have > received this in error, please advise the sender by reply e-mail and delete > the message immediately. You may also contact the LMH Process Improvement > Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be > secure or error-free as information could be intercepted, corrupted, lost, > destroyed, arrive late or incomplete, or contain viruses. The sender > therefore does not accept liability for any errors or omissions in the > contents of this message, which arise as a result of e-mail transmission. > Thank you. > > > > ------------------------------ > > Message: 2 > Date: Tue, 14 May 2013 13:33:21 -0400 > From: Richard Cartun <rcar...@harthosp.org> > Subject: [Histonet] Re: [IHCRG] p40 > To: "'ih...@googlegroups.com'" <ih...@googlegroups.com>, > "'histonet@lists.utsouthwestern.edu'" > <histonet@lists.utsouthwestern.edu>, Jean Taylor > <jtay...@meriter.com> > Message-ID: <51923d21020000770003a...@gwmail3.harthosp.org> > Content-Type: text/plain; charset="utf-8" > > I have been very impressed with BioCare's "p40" monoclonal predilute. > We are able to use it a 1:5 dilution on our Bond Max platform. > Excellent immunoreactivity so far! > > Richard > > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > >>>> "Taylor, Jean" <jtay...@meriter.com> 5/10/2013 1:53 PM >>> > > Hi everyone, > > I’m looking into ordering the p40 antibody for one of our pathologists. > He wants to use it to help distinguish between adenocarcinoma and > squamous cell ca in lung. I’m wondering what labs are using, the > monoclonal or polyclonal antibody, and where you purchase it from. I > haven’t found that a lot of companies that carry it. Any info would be > helpful. > > Thanks, > > Jean Taylor, HT(ASCP)QIHC > IHC Tech > Meriter Health Services > Madison, WI > > -- > -- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. The IHC Resource Group is a standing committee > within the National Society for Histotechnology. > > To post to this group, send email to ih...@googlegroups.com > To unsubscribe from this group, send email to > ihcrg+unsubscr...@googlegroups.com > For more options, visit this group at > http://groups.google.com/group/ihcrg?hl=en > > To contact the National Society for Histotechnology, email: > hi...@nsh.org or call 443.535.4060. > > --- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. > To unsubscribe from this group and stop receiving emails from it, send > an email to ihcrg+unsubscr...@googlegroups.com. > For more options, visit https://groups.google.com/groups/opt_out. > > > > ------------------------------ > > Message: 3 > Date: Tue, 14 May 2013 17:39:05 +0000 > From: Sarah Dysart <sdys...@mirnarx.com> > Subject: [Histonet] RE: Gross Room cleaner > To: Amber McKenzie <amber.mcken...@gastrodocs.net>, > "Histonet@lists.utsouthwestern.edu" > <Histonet@lists.utsouthwestern.edu> > Message-ID: > <f6f2daaf10c04c01899a85dc1463d...@blupr07mb001.namprd07.prod.outlook.com> > > Content-Type: text/plain; charset="us-ascii" > > Bleach =) > Just make sure to rinse after cleaning or they will rust > > Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie > Sent: Tuesday, May 14, 2013 11:29 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Gross Room cleaner > > > What do you guys use to clean your Gross Room utensils? I bought Lem'in Lift > from Mantek in the past to soak the rulers, scissors, tweezers, etc...Thanks! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 4 > Date: Tue, 14 May 2013 12:38:38 -0600 > From: Patsy Ruegg <pru...@ihctech.net> > Subject: RE: [Histonet] Re: [IHCRG] p40 > To: Richard Cartun <rcar...@harthosp.org>, ihcrg ihcrg > <ih...@googlegroups.com>, "Histonet@Lists. Edu" > <histonet@lists.utsouthwestern.edu>, jean taylor <jtay...@meriter.com> > Message-ID: <bay178-w11e21482da5d15f02aaea8d8...@phx.gbl> > Content-Type: text/plain; charset="Windows-1252" > > Richard now the ab companies are really going to hate you advocating diluting > predilute's further isn't that a sacrilege, what are we going to do with you, > no negs and now diluting predilutes. I am just kidding I do it all the time > myself. > Cheers, Patsy > > Date: Tue, 14 May 2013 13:33:21 -0400 > From: rcar...@harthosp.org > To: ih...@googlegroups.com; histonet@lists.utsouthwestern.edu; > jtay...@meriter.com > CC: > Subject: [Histonet] Re: [IHCRG] p40 > > I have been very impressed with BioCare's "p40" monoclonal predilute. > We are able to use it a 1:5 dilution on our Bond Max platform. > Excellent immunoreactivity so far! > > Richard > > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > >>>> "Taylor, Jean" <jtay...@meriter.com> 5/10/2013 1:53 PM >>> > > Hi everyone, > > I’m looking into ordering the p40 antibody for one of our pathologists. > He wants to use it to help distinguish between adenocarcinoma and > squamous cell ca in lung. I’m wondering what labs are using, the > monoclonal or polyclonal antibody, and where you purchase it from. I > haven’t found that a lot of companies that carry it. Any info would be > helpful. > > Thanks, > > Jean Taylor, HT(ASCP)QIHC > IHC Tech > Meriter Health Services > Madison, WI > > -- > -- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. The IHC Resource Group is a standing committee > within the National Society for Histotechnology. > > To post to this group, send email to ih...@googlegroups.com > To unsubscribe from this group, send email to > ihcrg+unsubscr...@googlegroups.com > For more options, visit this group at > http://groups.google.com/group/ihcrg?hl=en > > To contact the National Society for Histotechnology, email: > hi...@nsh.org or call 443.535.4060. > > --- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. > To unsubscribe from this group and stop receiving emails from it, send > an email to ihcrg+unsubscr...@googlegroups.com. > For more options, visit https://groups.google.com/groups/opt_out. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 5 > Date: Tue, 14 May 2013 14:06:55 -0500 > From: "Renz, Jason" <jkr...@primecare.org> > Subject: [Histonet] Bone marrow aspirate iron stain > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <1e0e2b14c709174b8ac2be0ae7f76833a2f451e...@exchange2k7.staprimecare.org> > > Content-Type: text/plain; charset="iso-8859-1" > > Hello, We are currently having problems with our bone marrow aspirate slides > for iron stains which we run on the Ventana nexes special stainer. The RBCs > look crenated or damaged. We currently fix them 5 minutes in methanol then > air dry before running them on the stainer. Any suggestions? > Jason Renz > HT(ASCP)/lab safety officer > St. Alexius Medical Center > 900 East Broadway > Bismarck ND, 58506 > (701)530-6733 > > ________________________________ > This email may include confidential and privileged information. If this is > not intended for your use, please destroy immediately and contact the sender > of the message. > > > > ------------------------------ > > Message: 6 > Date: Tue, 14 May 2013 15:33:41 -0400 > From: Eleonora Guadagnin <guadagnin.eleon...@gmail.com> > Subject: [Histonet] Gomori Trichrome Staining > To: histonet@lists.utsouthwestern.edu > Message-ID: > <cakfuly+wvzlxoxlpbdquec8rq7pbxd-q10ttsu9gv_4uima...@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hello everybody, > I am performing the Gomori Trichrome staining on frozen muscle sections > (10um) but I have couple of issues: > 1. The green staining is TOO dark; > 2. When the green is not too dark, all my muscle fibers are red. > > I checked the pH of the Gomori solution, is 2.15. > > If anyone has some advice, please!!!! > Thanks a lot! > > Eleonora > > > ------------------------------ > > Message: 7 > Date: Tue, 14 May 2013 18:35:30 -0400 > From: Paula Sicurello <pat...@gmail.com> > Subject: Re: [Histonet] Gross Room cleaner > To: Amber McKenzie <amber.mcken...@gastrodocs.net> > Cc: "Histonet@lists.utsouthwestern.edu" > <Histonet@lists.utsouthwestern.edu> > Message-ID: > <CAPSjddaaREpPsrbZysAATAHeWzW7xdr58-i3ekASg7=lfnb...@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Dispatch works well too. > > Paula > -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory > Duke University Health System > Rm.#251M, Duke South, Green Zone > Durham, North Carolina 27710 > P: 919.684.2091 > > On Tue, May 14, 2013 at 12:28 PM, Amber McKenzie < > amber.mcken...@gastrodocs.net> wrote: > >> >> What do you guys use to clean your Gross Room utensils? I bought Lem'in >> Lift from Mantek in the past to soak the rulers, scissors, tweezers, >> etc...Thanks! >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 8 > Date: Tue, 14 May 2013 23:01:06 +0000 > From: "Tony Henwood (SCHN)" <tony.henw...@health.nsw.gov.au> > Subject: [Histonet] RE: Bone marrow aspirate iron stain > To: "'Renz, Jason'" <jkr...@primecare.org>, > "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: <6d6bd1de8a5571489398b392a38a71579d25b...@xmdb04.nch.kids> > Content-Type: text/plain; charset="us-ascii" > > Hi Jason, > I am not familiar with the stainer but is the procedure starting from water > (not dewaxing & re-hydration as is used for paraffin sections)? > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Renz, Jason > Sent: Wednesday, 15 May 2013 5:07 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Bone marrow aspirate iron stain > > Hello, We are currently having problems with our bone marrow aspirate slides > for iron stains which we run on the Ventana nexes special stainer. The RBCs > look crenated or damaged. We currently fix them 5 minutes in methanol then > air dry before running them on the stainer. Any suggestions? > Jason Renz > HT(ASCP)/lab safety officer > St. Alexius Medical Center > 900 East Broadway > Bismarck ND, 58506 > (701)530-6733 > > ________________________________ > This email may include confidential and privileged information. If this is > not intended for your use, please destroy immediately and contact the sender > of the message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. If > you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Sydney Children's > Hospitals Network. > > This note also confirms that this email message has been virus scanned and > although no computer viruses were detected, The Sydney Childrens Hospital's > Network accepts no liability for any consequential damage resulting from > email containing computer viruses. > ********************************************************************************* > > > > ------------------------------ > > Message: 9 > Date: Tue, 14 May 2013 23:24:04 +0000 > From: Sebree Linda A <lseb...@uwhealth.org> > Subject: [Histonet] RE: Lysol IC for Ventana equipmetn cleaner > To: Amber McKenzie <amber.mcken...@gastrodocs.net>, > "Histonet@lists.utsouthwestern.edu" > <Histonet@lists.utsouthwestern.edu> > Message-ID: > <77dd817201982748bc67d7960f2f76af049...@uwhc-mbx12.uwhis.hosp.wisc.edu> > > Content-Type: text/plain; charset="us-ascii" > > The dilution is listed on the _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
