Re: [Histonet] [External] Re: Agarose embedded tissue arrays embedded in paraffin block

2024-05-20 Thread Otto, Christopher M via Histonet
, 2024 at 2:32 PM Jay Lundgren via Histonet mailto:histonet@lists.utsouthwestern.edu>> wrote: Where did the agarose come from? On Fri, May 17, 2024 at 12:09 PM Otto, Christopher M via Histonet < histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> wrot

Re: [Histonet] [External] Re: Agarose embedded tissue arrays embedded in paraffin block

2024-05-17 Thread Otto, Christopher M via Histonet
se come from? On Fri, May 17, 2024 at 12:09 PM Otto, Christopher M via Histonet mailto:histonet@lists.utsouthwestern.edu>> wrote: Hello everyone! I'm having trouble sectioning tissue array blocks where the array is in agarose embedded into a paraffin block. I've chilled the blocks a

Re: [Histonet] Agarose embedded tissue arrays embedded in paraffin block

2024-05-17 Thread Otto, Christopher M via Histonet
Hello everyone! I'm having trouble sectioning tissue array blocks where the array is in agarose embedded into a paraffin block. I've chilled the blocks and I'm sectioning on a rotary microtome, at 5 microns, with a high profile Accuedge blade. The paraffin surrounding the agarose sections