[Histonet] IHC Staining

Is anyone having a problem with red chromogen verses brown staining on HMB-45. We are having inconsistent staining with the red on HMB-45. Judith Pardue Histology Supervisor CHI Memorial Health Care Chattanooga, Tn. judith_par...@memorial.org This email and attachments contain information that

[Histonet] Tissue falling off positive slides

WE are having trouble with tissue coming off our he slides. Our heater is set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue positive slides. Judith Pardue CHI Memorial Chatt. Tn. 37343 judith_par...@memorial.org This electronic mail and any attached documents are intended

[Histonet] Fungus contamination

Having a problem with fungus on top of both the pas and gms stain. There are no solutions used on both test. Other than distilled water we can not figure it out. All solutions have been changed and clean containers used. Judith Gale Pardue Histology Supervisor judith_pardue@memorial .org

[Histonet] Water rinse for HE's

Does anyone use hot water rinses on their he slides. If we use cold water our slides are very light and inconsistent. We stain on an automatic stainer. Judith Pardue Histology Supervisor Memorial Health Care System judith_par...@memorial.org This electronic mail and any attached documents are

[Histonet] Negative Controls

Does anyone know of any articles talking about not using negative controls when using a polymer based detection system. Judith Pardue Memorial Hospital Chattanooga, Tn. judith_par...@memorial.org This electronic mail and any attached documents are intended solely for the named

[Histonet] Negative Control

At the first of August Richard Cartun posted that CAP had a new negative control policy. Does anyone know if it is already in effect and if it would apply to up coming inspections. Judith Pardue Histology Supervisor Memorial Hospital Chattanooga, Tn. judith_par...@memorial.org This

[Histonet] Peloris

Has anyone had problems with Peloris processors shuting down after a power outage. Both our processors shut down when the power went off last night and they were connected to a USP and plugged into an emergency outlet. This is the third time this has happened and we have had to have a service

[Histonet] Tissue Loss

I would like some input on a situation I am faced with in our lab. We are constantly having tissue coming off the slide on GI bx's. This is coming from one tech, she is a senior tech and takes offense to questioning her about the problem. From what I can tell all of the techs are following the

[Histonet] Methenamine

I have tried to order methenamine and was told they no longer make it. We have tried many different companies that have it listed in their products but when you order it you are told it is no longer available. Does anyone know where we can get it or what is being used in its place. Judith Gale

[Histonet] Leica Bond verses the Ventana Ultra

Need to know histotechs opinion of the Leica Bond verses the Ventana Ultra. judith_par...@memorial.org Judith Pardue This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. Sections 2510-2521, and contain information intended for the

[Histonet] Pulling apart sections

We use a teasing needle to pull apart our section. Jusith Pardue Memorial Health Care System Chattanooga, Tn. 37404 This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. Sections 2510-2521, and contain information intended for the

[Histonet] waterbaths

Does anyone know if a waterbath temp is set to low ( 37C degrees) if the staining will be effected by this. Judith Pardue Memorial Healthcare System Chattanooga,Tn. judith_par...@memorial.org This message and accompanying documents are covered by the Electronic Communications Privacy Act 18

[Histonet] Negative Pressure Rooms

Our histology lab is negative pressure and the hospital is remolding and wants the department to use one door to enter and exit the department.This will mean we will have to do that via and outside breezeway. Our concern is will this cause problems with our staining due to humidity or moisture

[Histonet] Immuno Assessments

Has anyone had any experience with an Immuno survey group out of Denmark named NordiQC. Judith Pardue Memorial Health Care System Chattanooga,Tn. 37404 This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. Sections 2510-2521, and contain