I have done just what you are talking about using Aclar. It is a plastic
embedding film (purchased from EMS). It works great for us.
Margaret E. Bisher
Electron Microscopy Histology Core Facility Manager
Department of Molecular Biology
Princeton University
Moffett Laboratory, Room 113
Princeton,
I have a pretty old microtome to donate. It is a Leitz 1512. I also have
some of those big blades that it uses. All you have to do is pay for the
shipping.
Cheers, Maggie
Margaret E. Bisher
Electron Microscopy Histology Core Facility Manager
Department of Molecular Biology
Princeton
I had a group here who were having trouble with their cryo-sections sticking
to the slides. Their samples were rat brains. He found that the Gold Super
Frost Plus worked well with in-situ but not for immuno. At least that is the
comment he wrote down and put on our bulletin board in the lab. Most
Hi Histonetters,
I have someone here who is having problems with their samples (mouse lung
and mammary). It appears that the sample is very brittle when she cuts and
in addition, some of the paraffin around the sample in the cassette is very
white in color instead of the normal translucent color.
Actually I had a project to find bacteria in the ovaries of drosophila. The
bacteria looked a lot like mitochondria and about the same size. That was a
lot of fun, but at least I got to use a TEM for the imaging. You could
barely see the sample in the dissecting microscope and it was only after I
We have a bottle of it here in the lab. It came from Acros, which means we
ordered it from Fisher Scientific.
Maggie
On 2/25/09 11:30 AM, Janice Mitchell mitchel...@email.chop.edu wrote:
Hi histoland, Anyone out there using canada balsam? If so where are
you purchasing? Thanks in advance.
Liquid nitrogen (-196C) and nitrogen slush (-207) are the coldest cryogens
used, but they have the slowest freezing rates. The most effective cryogens
are those that are those that stay a liquid when cooled almost to the
temperature of liquid nitrogen.
Propane (-189.6C mp) or Ethane (183.5C mp)
We (Princeton University) are also on a 37.5 hour week. That takes into
account 30 minutes for a lunch break.
Peggy
On 12/10/08 11:03 AM, [EMAIL PROTECTED] [EMAIL PROTECTED] wrote:
Because that is the length of time for a standard work week in this province.
Bryan
Why are times
I have seen them there! Mostly SEM's.
On 11/14/08 12:52 PM, Jackie M O'Connor Jackie.O'[EMAIL PROTECTED]
wrote:
Could you imagine these things on Ebay?
Joe [EMAIL PROTECTED]
Sent by: [EMAIL PROTECTED]
11/14/2008 11:21 AM
To
histonet@lists.utsouthwestern.edu
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Subject
Actually I belong to the MSA and they have a place on their web page to list
equipment for sale. If you know exactly what you have, then that would be
the way to go. You will have to join though, to post it. It doesn't cost
anything to join, just some information.
Margaret E. Bisher
Electron
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