Hello everyone,
We are trying to stain alpha smooth muscle actin (on mouse/human liver) using
the A5228 Sigma antibody and are getting nuclear staining while this stain
should be cytoplasmic. Does anyone have experience with nuclear staining of
alphaSMA? Is there anything I can do to
Hi all,
I would like to do an immunofluorescent double labeling with two
antibodies but 1 antibody works on acetone fixed frozen tissue but not
on formalin fixed paraffin embedded tissue (CD31 BD pharmingen 553370)
and the other one works on formalin fixed paraffin embedded tissue but
not on
for embedding in paraffin or should I use a
specific protocol manually and if so, which?
Thanks!
Phebe
From: Adam . [mailto:anonwu...@gmail.com]
Sent: Tuesday, 23 February 2010 10:52 AM
To: Phebe Verbrugghe
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet
Hi Adam,
Thanks a lot, might just give it a go on just formalin fixed tissue
first.
Phebe
From: Adam . [mailto:anonwu...@gmail.com]
Sent: Tuesday, 23 February 2010 12:01 PM
To: Phebe Verbrugghe
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet
