[Histonet] Corpora amylacea are destroyed after antigen retrieval?

2014-02-18 Thread Pirici Daniel
Has anyone observed corpora amylacea on brain tissue being destroyed after antigen retrieval (for example microwaving in citrate buffer)? We are experiencing this problem, as we are interested to study these structures, and we are not sure if it is a common artifact when preparing slides for

Re: [Histonet] Embedding paraffin for brain tissue?

2014-01-02 Thread Pirici Daniel
Hi there,   1. Have you changed anything in the processing and embedding processes themselves (dehydration, clearing agents and times, etc)? Friable sections could also come from extended times in the clearing agent...   2. There is also a section transfer system with a waterfall. We have a

[Histonet] A neuronal membrane marker?

2012-07-07 Thread Pirici Daniel
Hi to all Histoneters, I am searching for some while now for a good antibody against a specific neuronal membrane target. Also, it should “see” the neurites’ membranes, not only the perykarions'.. I know tubulin III and NF are very good, but for cytoskeleton. Anti-acetylcholine receptors and