[Histonet] Re: Staining Protocol

2010-08-19 Thread Poole, Kimberly
Hello Histonetters, I perform many HE staining and I think that the previous person who ran the lab, might be missing steps in his staining protocol. Would anyone be willing to share their protocols with me? Thanks for your help! Kimberly Poole B.Sc Casualty Management Section |

[Histonet] Brain Problems

2010-07-27 Thread Poole, Kimberly
Hi there, I am currently using rat brain for histology but something is not working. I follow the protocol below for tissue processing. Station Solution Routine (mins) 1 Formalin 10 2 Formalin 10 3 70% Ethanol 60 4 80% Ethanol 240 5 96% Ethanol 240 6 100%

[Histonet] Acid/Alcohol

2009-11-17 Thread Poole, Kimberly
Good Morning, I have a question regarding some histology Methods. I am about to order acid/alcohol and I am not sure why I am using it and what percentage I should get (1%, 5%, 0.5%). I also have a question regarding the staining procedure itself. Our staining procedure has about 27 steps

[Histonet] Histology Training

2009-03-31 Thread Poole, Kimberly
Hi, Does anyone know of a place that I can get histology training? I am specifically looking for a place in the US or Canada to go to for a couple of weeks. I am not able to take courses that are 1 to 2 years long. Thanks in advance!! Kimberly Poole B.Sc Casualty Management Section |