[Histonet] IHC wash buffer temperature?

Hello Histonetters, I work in research and we get too much non-specific background on our IHC-s. Anybody tried using wash solution at 37C instead of at room temperature? If not, why? Is it a bad idea? Thanks, Hanna Preiszner ETSU Pathology ___ Histonet

Re: [Histonet] ISO tissue with H. pylori for positive control

Thank you for all the generous offers to send me control tissue! I really appreciate the help of this group! Hanna Preiszner ETSU Pathology From: Preiszner, Johanna Sent: Friday, October 11, 2019 12:10 PM To: histonet@lists.utsouthwestern.edu Subject: ISO tissu

[Histonet] ISO tissue with H. pylori for positive control

Netters, could someone send us some H.pylori+ stomach to be used as positive control? I can offer Blastomyces populated human lung tissue for fungus control. We are small lab now, working for city clinics. Thanks! Hanna Preiszner ___ Histonet mailing li

[Histonet] How do you section PPFE tissue in embedding rings?

Hi, I received a lot of specimens embedded in plastic embedding rings...have a Leica RM2135 microtome. How can I put these blocks in the clamps of the microtome? Searched Internet with zero success. Nobody in town ever used embedding rings so I can not borrrow an adapter if that's what I need

[Histonet] StatLab paraffins?

Hi, does anybody use StatLab paraffin for embedding? If so which one, and what is your experience using it? Their pricing looks good, so I'd like to try. Thanks, Hanna Preiszner ETSU/QCOM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu h

[Histonet] cadaver tissue processing for histology

Hi, I got some cadaver tissue floating in "LTP embalming fluid w/ phenol". Can I put those straight into the processor? Or has to be rinsed/soaked before? If so what should I use? My Internet searches did not bring up anything useful. Thank you! Hanna Preiszner ETSU/QCOM Dept of Pathology __

[Histonet] anybody familiar with molecular sieves?

Hi, we recycle our ethanol and get back a lot of 92-93% ethanol. I looked up molecular sieves/ desiccants and what I see these can be used to selectively adsorb water from water/ethanol mixtures and the sieve/desiccant can be regenerated and used multiple times. If it worked we could save a

[Histonet] buffered zn-formalin recipe needed

Hi, For the life of me I can't locate a recipe for buffered zn-formalin, nor online or off. Could someone please help us out? Thanks, Hanna Preiszner ETSU/QCOM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwest

[Histonet] using FFPE positive control for frozen tissue IHC?

Hi, We have difficulty obtaining frozen tissue to use as positive control when we do IHC on frozen sections... Is it allowed to use FFPE sections for this purpose? If not, can someone explain to me why not? Is it wrong by principle or prohibited by regulations? If it's the regulations, can som

Re: [Histonet] Who said Natura maxime miranda in minimis. or Natura in minima maxima. who said this? Nature is the greatest in the smallest. ?

Plini, Hist. Nat. XI. 1. The qoute below is the simplified form of the original: Quum rerum natura nusquam magis, quam in minimis, tota sit. From: Jorge A. Santiago-Blay via Histonet [histonet@lists.utsouthwestern.edu] Sent: Sunday, June 12, 2016 4:06 PM

[Histonet] desperately in need of human alpha-synuclein positive control

Hi, anybody heard about a place where I can buy human alpha-synuclein IHC positive control slides? Or anybody can spare some? Or brains with Lewy bodies? I can offer other control blocks you might need. Thanks, Hanna Preiszner ETSU/QCOM ___ Histone