Re: [Histonet] problem with DAB staining

2013-03-07 Thread Robert Schoonhoven
We would need to see the entire set of steps as there may be a variety of causes. Robert Schoonhoven, HT/HTL (ASCP) From: Nicole Cosenza ncose...@siumed.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 7, 2013 12:50 PM Subject: [Histonet

Re: [Histonet] Immuno Bed Plastic Question

2013-02-13 Thread Robert Schoonhoven
and then start your IHC procedure. Robert Schoonhoven, HT/HTL (ASCP) From: Allyse Mazzarelli allyse...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 13, 2013 1:19 PM Subject: [Histonet] Immuno Bed Plastic Question Hi histonetters! I

Re: [Histonet] Process and hold?

2013-02-12 Thread Robert Schoonhoven
.  As the tissues are processed through paraffin they are protected and will not dry out. Robert Schoonhoven, HT/HTL (ASCP) From: Tom McNemar tmcne...@lmhealth.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, February 12, 2013 1

[Histonet] Looking for Histology work in the Newark DE area

2013-01-11 Thread Robert Schoonhoven
...@gmail.com   Robert Schoonhoven, HT/HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] staining site of bone deposition on live specimens

2013-01-03 Thread Robert Schoonhoven
In the past we used tetracycline on rats.  Not sure how that would affect live aquatic amphibians.       Robert Schoonhoven, HT/HTL (ASCP) From: Christopher Sheil csh...@jcu.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, January 3, 2013 2:23 PM

Re: [Histonet] GMAblocks

2009-09-08 Thread Robert Schoonhoven
to penetrate the polymerized GMA which cannot be etched. Robert Schoonhoven, HT/HTL (ASCP) From: Edwards, R.E. r...@leicester.ac.uk To: Histonet histonet@lists.utsouthwestern.edu Sent: Friday, September 4, 2009 11:29:29 AM Subject: [Histonet] GMAblocks Assuming

[Histonet] Looking for new and used equipment

2009-05-30 Thread Robert Schoonhoven
I am working as a consultant to a new (from scratch) histology lab in the Kentucky - Indiana area and we are looking at both new (some), and refurbished (mostly) equipment. I would appreciate an off list reply from suppliers. Thanks, Bob Robert Schoonhoven, HT/HTL (ASCP) robert_schoonho

[Histonet] looking for Ford Royer

2009-05-29 Thread Robert Schoonhoven
Please contact me off list. Thanks Robert Schoonhoven, HT/HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] BRDU, Tetracycline, calcein in bone

2009-04-21 Thread Robert Schoonhoven
You can also perform BrdU on the MMA sections the BrdU label is quite hardy. Robert Schoonhoven From: Nancy W. Troiano nancy.troi...@yale.edu To: histonet@lists.utsouthwestern.edu Sent: Tuesday, April 21, 2009 1:37:01 PM Subject: [Histonet] BRDU, Tetracycline

Re: [Histonet] brdu on ffpe rat fixed for up to 2 years in NBF

2009-04-20 Thread Robert Schoonhoven
Patsy, If the animal was treated with BrdU initially, then yes I have. Unfortunately All of my papers and reference materials are boxed up at the moment (preparing to move) but I have done this procedure so many times that I can pretty much reconstruct it from memory. 1) Hydrate slides to

Re: [Histonet] HISTOS 5 processor

2009-04-09 Thread Robert Schoonhoven
Ann, I was able to investigate this unit at Milestone Medical as their US headquarters is located locally here in Kalamazoo, MI. They have an excellent line of products and I recommend them highly. Disclaimer: I have no commercial interest in Milestone Medical. Regards, Bob Schoonhoven,

[Histonet] Travelling Histotechs

2009-04-09 Thread Robert Schoonhoven
I have noticed in the past several posts looking for traveling histo techs. Would one of these agencies please contact me off list? 35+ years of experience recently become available. Robert Schoonhoven HT/HTL (ASCP) 269-615-0576

Re: [Histonet] Removing PMMA blocks from glass vials

2009-04-03 Thread Robert Schoonhoven
Paul, Put in LN2 (or leave in -80 freezer for about 1 hour). Using the appropriate PPE, wrap in towel, hit with Hammer (gently...), welcome to use this SOP. Bob, Robert Schoonhoven From: Monfils, Paul pmonf...@lifespan.org To: histonet