I have tried this procedure multiple times and everytime the solution turns purple in the glassware filled with deionized water, even after rinsing in running water for 2+ minutes. We do not have a dishwasher, so all the glassware is hand-washed. Could there be a problem with our DI water? Has anyone else had an issue with this?
Thanks, Samantha Sackmann, CG(ASCP)CMHTLCM, Histotechnologist II CellNetix Pathology & Laboratories 800 West 5th Avenue Spokane, WA 99204 509-473-2311 sedwa...@cellnetix.com What is everyone using to test their glassware for detergent residue? Is there a ready to use solution or not? CAP Checklist... Glassware Cleaning Phase II There are appropriate documented procedures for handling and cleaning glassware, including methods for testing for detergent removal. NOTE: Special instructions for micropipettes, cuvets, acid washing, etc. must be included. A simple procedure to check for detergent residue uses bromcresol purple (0.1 g bromcresol purple in 50 mL ethyl alcohol). Pipette approximately 5 cm (2 inches) distilled water into a representative, washed, glassware item. Add two to three drops bromcresol solution. A purple color reveals residual detergent. A yellow color indicates satisfactory rinsing. Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mroark <@t> sfmc.net<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> http://www.sfmc.net<http://www.sfmc.net/> Samantha Sackmann, CG(ASCP)CMHTLCM, Histotechnologist II CellNetix Pathology & Laboratories 800 West 5th Avenue Spokane, WA 99204 509-473-2311 sedwa...@cellnetix.com DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
