[Histonet] a problem with giemsa stain for h.pylori

2013-01-06 Thread Shahram Sabeti
hello every body,     on giema staining of gastric tissue slides for h.pylori,we have encounterd a major problem for a while.the problem is deposition of large flakes on the mucosal surface and we do not know why? do you know how to get rid of this problem. thank you in advance for your kind

[Histonet] restaining method of wright-giemsa stained peripheral blood slides

2011-07-11 Thread Shahram Sabeti
dear fellows,     I have some precious blood slides at hand but with a major problem,pallor, because all of them are unmounted and a long time has elapsed from their preparation .their restaining was frightening for me because a fault could result in  their deterioration.    do you have any

[Histonet] congo red staining method for abdominal fat aspirates

2011-05-17 Thread Shahram Sabeti
dear colleagues, I have a problem with congo red staining of abdominal fat aspiration slides.i perform the same way as for slides prepared from paraffin-embedded blocks, but the results are  poor.     thank you in advance for your help.     sincerely, sabeti 

[Histonet] how to bleach melanoma cases without bad effect on IHC

2011-03-22 Thread Shahram Sabeti
hello dear fellows,    i have an IHC project on paraffin embedded melanoma cases; some of them being heavily pigmented. i have seen many protocols based on H2O2 to bleach the pigmented ones, but i am not sure about any possible effect on antigen retrieval.please guide me . yours, sabeti