[Histonet] RE:Antigen retrieval paraffin skin samples

2009-07-30 Thread Sorenson, Jon (Nampa)
We use and like the PASCAL pressure cooker system from DAKO, it is uniform, convenient and fairly Easy to use. Jon Jon Sorenson Histology Coordinator Mercy Medical Center jonsore...@chiwest.com 208-463-5267 Message: 17 Date: Wed, 29 Jul 2009 14:05:29 -0700 (PDT) From: "Nicholas David Evans"

[Histonet] RE: Histonet] xylene

2009-05-05 Thread Sorenson, Jon (Nampa)
Loretta, When I was using the CBG recycler at a former employer, we had a similar incident, because the drain was plugged when the waste container got too full and the paraffin solidified in the drain tube. Check that possibility. Jon Jon Sorenson Histology Coordinator Mercy Medical Center jo

[Histonet] Re: cactus spines

2009-02-19 Thread Sorenson, Jon (Nampa)
After embedding in the buttocks and having the biopsy taken (to include the spines) try soaking the faced blocks in NAIR hair remover. Might help, Jon Jon Sorenson Histology Coordinator Mercy Medical Center jonsoren...@chiwest.com 208-463-5267 Try embedding the spine in the buttocks of th

RE: RE: [Histonet] uncertified techs in Histology

2009-02-12 Thread Sorenson, Jon (Nampa)
In response to what Tom McNemar wrote: I too, am a certified HT,HTL for 25 plus years and strongly agree with Tom. I followed a B.S. in Biology/Chemistry with training in a 2 year A.S. program that included a 6 month stint as an unpaid intern at the Mayo Clinic in Rochester, MN. I guess I wi

[Histonet] RE: Procedure for Immunoperoxidase on a Frozen Section

2009-01-29 Thread Sorenson, Jon (Nampa)
This is how we handle Frozen sections and touch preps for IHC: Frozen tissue is picked up on "Plus" slides, the sections are air dried for a approximately 1 hour and then fixed in room temperature acetone, and finally air-dried again. These slides can now be used or stored in a freezer for late

[Histonet] Document Control

2009-01-29 Thread Sorenson, Jon (Nampa)
Does anyone in this group have any good ideas, either software or procedural that will do a good job of document control. Specifically: 1. Controlling who has access to editing and copies. 2. An automated review reminder process. 3. Electronic or online review and/or signature process. T

[Histonet] RE: Time & temp in oven prior to IHCs

2009-01-13 Thread Sorenson, Jon (Nampa)
70 C for 20-30 minutes, but beware of adding an adhesive to waterbaths if you are using silane treated (plus) slides, you may be creating an adhesion problem by doing so. Jon Jon Sorenson Histology Coordinator Mercy Medical Center jonsoren...@chiwest.com _

[Histonet] RE: Spit and Diastase

2009-01-13 Thread Sorenson, Jon (Nampa)
Is the person involved a smoker? We have had trouble with the saliva of people that smoke not having diastase in the past, Jon Jon Sorenson Histology Coordinator Mercy Medical Center jonsoren...@chiwest.com ___ Histo

[Histonet] RE: Histonet Digest, Vol 62, Issue 15

2009-01-13 Thread Sorenson, Jon (Nampa)
Is the person involved a smoker? We have had trouble with the saliva of people that smoke not having diastase in the past, Jon Jon Sorenson Histology Coordinator Mercy Medical Center jonsoren...@chiwest.com ___ Histo