[Histonet] Prion Scrapie IHC

Does anyone do the Scrapie Prion IHC testing on the Leica Bond? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Vet lab accreditation

NAHLN Sheryl Stephenson Histotechnologist NJ Department of Agriculture Animal Health Diagnostic Labs -Original Message- From: Johnson, Carole via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, July 23, 2015 5:14 PM To: histonet@lists.utsouthwestern.edu Subject:

Re: [Histonet] Leica bond

So far so good. Been using it for a year now. No issues or grips that couldn't be worked out with Leica. They have been very supportive. Their field staff comes to assist you from start to finish if need be. Leica even has classes to train on their machine in IL; all expenses paid. Oh

[Histonet] lab math: ihc dilution

To all you math whiz out there, please help with this math dilution. If my Ab conc is 1.56 mg/mL and they want to optimize to 0.8 ug/mL, what dilution should I use? Thanks, Sheryl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Rabies tissue Fixed in Formalin.

Hi, Does anyone have any knowledge and literature on whether or not 10%NBF inactivates rabies in animal tissue during fixation? Thanks, Sheryl. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] Edit protocol for Shandon Gemini Varistainer

Lol, I had the same problem. Yes the manual does not thoroughly explain this. U have to delete the protocol from the active stains list, edit the protocol and make sure reagents are linked to the correct buckets, then add the protocol back to the active stain list. I'm not sitting in front

[Histonet] HT HistoDeck question...

Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c)

RE: [Histonet] HT HistoDeck question...

Thank you All! Sheryl Stephenson | Histology Technician   -Original Message- From: Bernice Frederick [mailto:b-freder...@northwestern.edu] Sent: Thursday, October 03, 2013 9:43 AM To: Watson, Linda; Lee Peggy Wenk; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: RE

RE: [Histonet] Possible continuing automatic staining problem

What do you mean by muted; can you give a little more description? And what type of tissue, and is this HE? Sheryl Stephenson | Histology Technician -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

RE: [Histonet] ASCP HT exam

C*O*N*G*R*A*T*U*L*A*T*I*O*N*S!! woohoo. Sheryl Stephenson | Histology Technician   -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bharti Parihar Sent: Thursday, June 14, 2012 6:03 PM To:

RE: [Histonet] microtome

If you want to stay with Leica; they have a newer model called RM2255 it has the manual and automatic rotations. Sheryl Stephenson | Histology Technician   -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Reagent rotation

We do it by the number of Runs. About every 6 run for rotations and 12 runs for a full change out. But we also have another processor as well. Sheryl Stephenson | Histology Technician Main 908.947.1100 Fax908.947.1085 Direct: 908.947.1624 

RE: [Histonet] Embedding

That was my initial thought, check your paraffin. I could be coming from the paraffin.. or whatever you are cleaning with make sure its dust free/debris free. Sheryl Stephenson | Histology Technician Main 908.947.1100 Fax908.947.1085 Direct:

RE: [Histonet] Leitz 1512

Yeah, a brand new Leica RM 2255.:D Sheryl Stephenson | Histology Technician Main 908.947.1100 Fax908.947.1085 Direct: 908.947.1624 sstephen...@lifecell.com www.lifecell.com LifeCell Corporation | One

RE: [Histonet] Cassett Labeler

We use PrintMate by Thermo Fisher. Kinda loud by its pretty neat. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Tuesday, May 08, 2012 2:57 PM To: histonet@lists.utsouthwestern.edu

RE: [Histonet] how often do you change your hematoxylin?

Hi Meryl, What stain are you using your Hematoxylin for? We use Harris Hematoxylin for HE staining and we filter it each day that we need to use it.We change it once per month. We are also not a high volume lab. We are a histology department in a biotech company. Your suspicions are