[Histonet] Kappa/Lambda ISH on Ventana

2011-03-31 Thread Steven Wilkes
Hi Histonet! I have been tasked with validating Kappa and Lambda on our Ventana Benchmark XT machines. Does anyone who uses this platform have suggestions as to which Ventana protease works best (1, 2 or 3)? Additionally, any other information concerning the optimization (suggested incubation

[Histonet] DOG1 (c-kit neg. GIST tissue)

2010-05-21 Thread Steven Wilkes
Hello We are interested in validating DOG1, but while the literature states 5-15% of GIST cases are c-kit negative, we are unable to obtain any. We currently use CD117, but would add DOG1 to our library if we could validate that it is a more sensitive marker - and to do so we'd need c-kit

[Histonet] Glypican-3 on BondMax

2009-06-18 Thread Steven Wilkes
Hi Histonetters! Has anyone been using Glypican-3 on the Leica BondMax platform? If so, please msg me, as I have some questions. I am having a tough time finding the right conditions... dilution, pretreatment, incubation times etc.. thanks Steven srwil...@gmail.com

[Histonet] Glypican-3 on BondMax

2009-06-18 Thread Steven Wilkes
Hi Histonetters! Has anyone been using Glypican-3 on the Leica BondMax platform? If so, please msg me, as I have some questions. I am having a tough time finding the right conditions... dilution, pretreatment, incubation times etc.. thanks Steven srwil...@gmail.com