Has anyone used a disinfectant other than Lysol IC to do the decon on the
Ventana Ultras?
Thanks
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edumailto:shun...@beaumont.edu
Beaumont Hospital, Royal Oak MI. It's great!
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu
We do the same process as Linda does here at Beaumont.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun
Good morning
Does anyone have suggestions for antibodies for M2D2 and SMAD? We have not
been able to get these antibodies to work for us on the Ventana Ultras.
If you do have suggestions, protocols and control tissue suggestions would also
be appreciated.
Thanks
Sue
Sue Hunter, Supervisor
Try Victor Wong, Autostainer Technical Solutions 888-505-9545 cell
646-378-9222 www.autostainertech.com
Not sure if he is still in business, he was located in New Windsor NY
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun
Ventana Ultras and they are busy
all the time. and so are my 2 techs.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun
and exceptional service to
the NSH.
Please join us in congratulating Peggy on this well deserved recognition and
award.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edumailto:shun...@beaumont.edu
- this will be a really nice addition to the reviewing process. I
believe there are many laboratories where the cytotechs read out the urovsyions.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun
, (and use tap water) so I
don't think that is your problem. We are using the cheaper Fisher Superfrost
plus slides and they seem to work as well as the full priced ones. We cut our
immuno sections at 3um.
I know how frustrating this can be - I feel your pain!
Good luck!
Sue
Sue Hunter, Supervisor
to get consistent staining here. As they
say, The Enemy of Good is Better. Wish Leica had not made it better! It was
working very nicely before.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message
To: Sue Hunter; Robert Fauck [CCDHB]; histonet@lists.utsouthwestern.edu
Subject: RE: Her-2 antibody
Never had any such problems with either the Max or the Bond IIIs in the 6+
years we have had them(no matter how many labels are on the slide - we have had
three labels on a slide with no problems
to
running them on the Ultra.
Just my opinion.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu
RNA technique.
If you are making up your own master mixes and primer mixes, be sure to use
RNAse free water.
Good luck
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun
Hello
Just wanted to add one more thing - we actually use a dedicated pyrex dish
(maybe 6x10 inches) for our water bath for RNA sections. We use warm tap
water, but you can put it in the microwave for a short bit if it needs to be
warmer. You can spray the dish with RNAse away and wipe before
parameter of pressure to factor in to bring the solution
to a boil.
Good luck - hope it works for you.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
it as there was no interest by our clinicians.
If you ever find an antibody, please let me know. I would be interested in
trying it also.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun
Thanks for the update.
sue
From: Jim Burchette [mailto:jburc...@gmail.com]
Sent: Friday, March 15, 2013 11:02 AM
To: Sue Hunter
Cc: Zimmerman, Billie; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: SOS EGFRV3
Sue, you are correct, EGFR v III was developed at Duke by Dr Darell
We use Ventana's ready to use for the Ultra, but it is clone KP1. You can get
the same clone in a concentrate from Cell Marque.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun
.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Zimmerman,
Billie
Sent: Friday
only test FFPE tissues.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Susan Foreman
. You will have to try
dilutions and time - usually 10 minutes is good, but with a diluted solution
you may need to go longer. Then wash the slides in PBS to get rid of the H2O2.
Good Luck
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun
need more material.
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Orsini
Sent
It is my understanding that you can only bill for one 88342 on a specimen -
technical or professional, even if you perform the same stain on multiple
blocks of that specimen. For A1, A2, A3 etc you can only charge once. For A1,
B1, C1, etc you may charge a 88342 per specimen.
Sue
Sue Hunter
long time and
gives off a nice bright light.
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf
Can you use the 3M freezing fluid in a histobath instead of isopentane?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
abri...@brightinstruments.com
Sent: Tuesday, April 10, 2012 6:46 AM
To: Bob
pretreatments on each rack because of
timing/heating issues. But we have not found either of these two issues to be
a problem for us. The Bond III is supposed to be even faster than the Bond Max
but haven't looked at that.
Sue
Sue Hunter
Supervisor, Advanced Diagnostics
Beaumont Health Systems
that take
longer than the immunos.
sue
-Original Message-
From: Rathborne, Toni [mailto:trathbo...@somerset-healthcare.com]
Sent: Thursday, April 05, 2012 10:51 AM
To: Sue Hunter; Wellen, Terrence D. :LPH Lab; histonet@lists.utsouthwestern.edu
Subject: RE: Leica Bond IHC Platform
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