Monica -
The Klymkowsky lab has a trove of Xenopus protocols:
http://klymkowskylab.colorado.edu/
Their wax and cryo page also has the reference to the 1989 Methods in Cell
Biology paper covering Xenopus histological methods.
If you can, leave the oocytes in the ovary - it is MUCH easier to
Several labs here use 21st Century Biochemicals for custom
peptides:
http://www.21stcenturybio.com/
Their tech support people are VERY helpful.
Good luck!
Tamara
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Tamara Howard
Cell Biology & Physiology
UNM-HSC
Albuquerque, NM
***
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I'm not certain that this will fix your problem, but I'd
ditch the Tween in the final wash or two before mounting.
There may be too much residual buffer on your slides when
you add the Prolong - I wick off as much as I can, without
drying the tissue, before mounting.
You let the Prolong cure
Apologies to everyone on the cross-post for this question,
but I'm looking for feedback on this image analysis
package (Definiens Tissue Studio). One of our faculty
members is interested in it & I know nothing about it,
other than what the company website has to offer; I'd
rather hear from peo
