[Histonet] IHC on paraffin sections

Dear All, I want to stain for inos, Dectin-1 and Hem OX-1 on formalin fixed paraffins sections. I was told by some one these antibodies only work on frozen sections and not on paraffin sections, but I see all these antibodies datasheet say it work on paraffin sections as well. Any input will

[Histonet] Immunofluorescence

Dear All, Can some one please send the protocol for immunofluorescence, I want to stain for FITC CD45.1 on mice spleens. Thank you very much for your help. Many Thanks, Anil kumar. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] CD45.1 CD45.2 IF on parrafin sections.

Dear All, I want to do immunofluorescence on formalin fixed paraffin sections, Can you guys help me sending protocol how to do it ? Thank you very much for your help. BW Anil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Endothelial histochemistry

Hi Ali, I tried FITC cd31 on mouse liver frozen section with tumors it worked really well. Good luck, Anil Kumar. On 22/10/10 11:29, Ali Nasr Esfahani alina...@student.liu.se wrote: Hi, I am just new in this group, so I hope this is the way I can get help. I am doing histochemistry on human

[Histonet] Necrotic and apoptotic cells

Dear All, I want to do look for Necrotic and apoptotic cells on liver tumor sections. What staining and antibodies are good for this technique?. Please let me know. Thank you very much in advance. Many Thanks, Anil Kumar. ___ Histonet mailing list

[Histonet] Slide expiry

Dear All, I purchased bulk of Slides polysin coated slides and on the box it say they expire by end of this year, Does it really effect ?? Or I don't have to bother. Please let me know. Many Thanks, Anil Kumar. ___ Histonet mailing list

[Histonet] no subject

Dear All, I have a PFA fixed liver frozen sections and I want to stain for cd45.1, cd45.2 and f4/80. Can you guys please help me with the protocols for the staining. I would like to do Immunofluroscence. Thank you all in advance. Many Thanks, Anil Kumar.

[Histonet] IHC and IF

Dear All, I have kind of funny problem. I am doing IHC (substrate reaction suing Dabs reagent) and IF ( direct labeling of my monoclonal antibody with flurochrome). I am working on mouse liver frozen sections. When I did IHC the staining looks like cytoplasm and if I do IF for the same protein

[Histonet] IHC mouse antibody on mouse tissue

Hi All, Does any one got a nice protocol to do IHC using mouse monoclonal antibody on mouse tissue. Either frozen or paraffin section. Please let me know. Thanks in advance. ANIL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] no subject

Hi All, I have a problem with immunohistochmesitry. I am have raised my own antibody and it is not commercially available. This monoclonal antibody works fine for western blot. When it come to immunohistochemistry on mice liver frozen section, it is binding every where. I can see staining in my