should be changed after each H&E run in my opinion.
> Adequate clean water rinses are important to not have carry over of clearant
> into bluing reagents or bluing reagent into eosin in order to maintain
> correct pH for staining.
>
> Good luck
>
> Gayle M. Callis
> H
You should be filtering your Hematoxylin on a daily basis regardless of what
the manufactures says. We use to filter twice a day since we did a traditional
overnight run and then again in the afternoon for specimens that had been
microwave processed. So much tissue washes off in the solutions
We run patient tissue as a negative and we use diluent as the reagent negative.
TH
> Message: 7
> Date: Fri, 17 Jul 2015 14:48:17 +
> From: "Abbott, Tanya"
> To: "histonet@lists.utsouthwestern.edu"
>
> Subject: [Histonet] IHC negative controls
> Message-ID:
>
> <852f7d2c14fb4
What is the allowable temperature range for a histology
specimen after collection in formalin for shipping and storage?
Any ideas?
Tim
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