Re: [Histonet] Hematoxylin Precipitate

2015-09-22 Thread Tim H via Histonet
should be changed after each H&E run in my opinion. > Adequate clean water rinses are important to not have carry over of clearant > into bluing reagents or bluing reagent into eosin in order to maintain > correct pH for staining. > > Good luck > > Gayle M. Callis > H

Re: [Histonet] Hematoxylin Precipitate

2015-09-22 Thread Tim H via Histonet
You should be filtering your Hematoxylin on a daily basis regardless of what the manufactures says. We use to filter twice a day since we did a traditional overnight run and then again in the afternoon for specimens that had been microwave processed. So much tissue washes off in the solutions

Re: [Histonet] IHC negative controls

2015-07-17 Thread Tim H via Histonet
We run patient tissue as a negative and we use diluent as the reagent negative. TH > Message: 7 > Date: Fri, 17 Jul 2015 14:48:17 + > From: "Abbott, Tanya" > To: "histonet@lists.utsouthwestern.edu" > > Subject: [Histonet] IHC negative controls > Message-ID: > > <852f7d2c14fb4

[Histonet] Allowable temperature range

2015-04-01 Thread Tim H
What is the allowable temperature range for a histology specimen after collection in formalin for shipping and storage? Any ideas? Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists